LOCUS       BBa_J06134              2081 bp    DNA     linear       12-May-2024
ACCESSION   BBa_J06134
VERSION     BBa_J06134.1  
FEATURES             Location/Qualifiers
     misc_feature    1..35
                     /label=P(Bla)
     promoter        25..30
                     /label=-10
     promoter        4..9
                     /label=-35
     promoter        1..35
                     /label=BBa_I14018
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..778
                     /label=lasR
     misc_feature    779..811
                     /label=LVA
     misc_feature    541..541
                     /label=G
     misc_feature    818..842
                     /label=Help:Barcodes
     CDS             62..817
                     /label=BBa_C0079
     stem_loop       862..905
                     /label=stem_loop
     misc_feature    851..930
                     /label=BBa_B0010
     terminator      851..930
                     /label=BBa_B0010
     stem_loop       946..965
                     /label=T7 TE
     misc_feature    972..972
                     /label=stop
     polyA_site      966..979
                     /label=polya
     misc_feature    939..979
                     /label=BBa_B0012
     terminator      939..979
                     /label=BBa_B0012
     misc_feature    1033..1050
                     /label=OP2
     misc_feature    1093..1112
                     /label=OP1
     promoter        1104..1109
                     /label=-35
     promoter        1127..1132
                     /label=-10
     promoter        988..1144
                     /label=BBa_R0079
     RBS             1153..1163
                     /label=RBS-4\Weaker
     RBS             1159..1162
                     /label=RBS
     misc_feature    1153..1163
                     /label=BBa_B0033
     RBS             1153..1163
                     /label=BBa_B0033
     CDS             1173..1880
                     /label=cI lambda
     misc_feature    1881..1913
                     /label=LVA
     misc_feature    1920..1944
                     /label=Help:Barcodes
     CDS             1170..1919
                     /label=BBa_C0051
     stem_loop       1964..2007
                     /label=stem_loop
     misc_feature    1953..2032
                     /label=BBa_B0010
     terminator      1953..2032
                     /label=BBa_B0010
     stem_loop       2048..2067
                     /label=T7 TE
     misc_feature    2074..2074
                     /label=stop
     polyA_site      2068..2081
                     /label=polya
     misc_feature    2041..2081
                     /label=BBa_B0012
     terminator      2041..2081
                     /label=BBa_B0012
ORIGIN
        1 tgtaagttta tacataggcg agtactctgt tatggtacta gagaaagagg agaaatacta
       61 gatggccttg gttgacggtt ttcttgagct ggaacgctca agtggaaaat tggagtggag
      121 cgccatcctc cagaagatgg cgagcgacct tggattctcg aagatcctgt tcggcctgtt
      181 gcctaaggac agccaggact acgagaacgc cttcatcgtc ggcaactacc cggccgcctg
      241 gcgcgagcat tacgaccggg ctggctacgc gcgggtcgac ccgacggtca gtcactgtac
      301 ccagagcgta ctgccgattt tctgggaacc gtccatctac cagacgcgaa agcagcacga
      361 gttcttcgag gaagcctcgg ccgccggcct ggtgtatggg ctgaccatgc cgctgcatgg
      421 tgctcgcggc gaactcggcg cgctgagcct cagcgtggaa gcggaaaacc gggccgaggc
      481 caaccgtttc atagagtcgg tcctgccgac cctgtggatg ctcaaggact acgcactgca
      541 aagcggtgcc ggactggcct tcgaacatcc ggtcagcaaa ccggtggttc tgaccagccg
      601 ggagaaggaa gtgttgcagt ggtgcgccat cggcaagacc agttgggaga tatcggttat
      661 ctgcaactgc tcggaagcca atgtgaactt ccatatggga aatattcggc ggaagttcgg
      721 tgtgacctcc cgccgcgtag cggccattat ggccgttaat ttgggtctta ttactctcgc
      781 tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct agtgtagatc
      841 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      961 gggcctttct gcgtttatat actagaggcc cctcgctgag cgcgtcccgg agctgggggc
     1021 aacctagctg ccacctgctt ttctgctagc tattccagcg aaaacataca gatttccggc
     1081 gaaatcaagg ctacctgcca gttctggcag gtttggccgc gggttctttt tggtacacga
     1141 aagctactag agtcacacag gactactaga tgagcacaaa aaagaaacca ttaacacaag
     1201 agcagcttga ggacgcacgt cgccttaaag caatttatga aaaaaagaaa aatgaacttg
     1261 gcttatccca ggaatctgtc gcagacaaga tggggatggg gcagtcaggc gttggtgctt
     1321 tatttaatgg catcaatgca ttaaatgctt ataacgccgc attgcttgca aaaattctca
     1381 aagttagcgt tgaagaattt agcccttcaa tcgccagaga aatctacgag atgtatgaag
     1441 cggttagtat gcagccgtca cttagaagtg agtatgagta ccctgttttt tctcatgttc
     1501 aggcagggat gttctcacct gagcttagaa cctttaccaa aggtgatgcg gagagatggg
     1561 taagcacaac caaaaaagcc agtgattctg cattctggct tgaggttgaa ggtaattcca
     1621 tgaccgcacc aacaggctcc aagccaagct ttcctgacgg aatgttaatt ctcgttgacc
     1681 ctgagcaggc tgttgagcca ggtgatttct gcatagccag acttgggggt gatgagttta
     1741 ccttcaagaa actgatcagg gatagcggtc aggtgttttt acaaccacta aacccacagt
     1801 acccaatgat cccatgcaat gagagttgtt ccgttgtggg gaaagttatc gctagtcagt
     1861 ggcctgaaga gacgtttggc gctgcaaacg acgaaaacta cgctttagta gcttaataac
     1921 gctgatagtg ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca
     1981 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     2041 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//