LOCUS       BBa_J06135              1092 bp    DNA     linear       13-May-2024
ACCESSION   BBa_J06135
VERSION     BBa_J06135.1  
FEATURES             Location/Qualifiers
     misc_feature    1..151
                     /label=R0079
     misc_feature    152..168
                     /label=CI lambda O1
     promoter        117..122
                     /label=-35
     promoter        140..145
                     /label=-10
     promoter        1..168
                     /label=BBa_I14016
     RBS             177..187
                     /label=RBS-4\Weaker
     RBS             183..186
                     /label=RBS
     misc_feature    177..187
                     /label=BBa_B0033
     RBS             177..187
                     /label=BBa_B0033
     CDS             194..955
                     /label=YFP (LVA)
     misc_feature    950..955
                     /label=2
     misc_feature    911..949
                     /label=SsrA
     misc_feature    435..435
                     /label=A (Q->L)
     misc_feature    194..955
                     /label=BBa_E0032
     misc_feature    194..955
                     /label=BBa_E0032
     stem_loop       975..1018
                     /label=stem_loop
     misc_feature    964..1043
                     /label=BBa_B0010
     terminator      964..1043
                     /label=BBa_B0010
     stem_loop       1059..1078
                     /label=T7 TE
     misc_feature    1085..1085
                     /label=stop
     polyA_site      1079..1092
                     /label=polya
     misc_feature    1052..1092
                     /label=BBa_B0012
     terminator      1052..1092
                     /label=BBa_B0012
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtaca ctacctctgg cggtgatata ctagagtcac
      181 acaggactac tagatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
      241 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
      301 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      361 gccctggccc accctcgtga ccaccttcgg ctacggcctg caatgcttcg cccgctaccc
      421 cgaccacatg aagctgcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      481 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
      541 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      601 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
      661 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
      721 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
      781 gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg
      841 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
      901 gctgtacaag aggcctgctg caaacgacga aaactacgct ttagtagctt aataatacta
      961 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1021 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     1081 tctgcgttta ta
//