LOCUS       BBa_J06137              1785 bp    DNA     linear       13-May-2024
ACCESSION   BBa_J06137
VERSION     BBa_J06137.1  
FEATURES             Location/Qualifiers
     misc_feature    1..151
                     /label=R0079
     misc_feature    152..168
                     /label=CI lambda O1
     promoter        117..122
                     /label=-35
     promoter        140..145
                     /label=-10
     promoter        1..168
                     /label=BBa_I14016
     misc_feature    181..184
                     /label=conserved
     RBS             177..188
                     /label=BBa_B0034
     CDS             195..956
                     /label=YFP (LVA)
     misc_feature    951..956
                     /label=2
     misc_feature    912..950
                     /label=SsrA
     misc_feature    436..436
                     /label=A (Q->L)
     misc_feature    195..956
                     /label=BBa_E0032
     misc_feature    195..956
                     /label=BBa_E0032
     RBS             965..975
                     /label=RBS-4\Weaker
     RBS             971..974
                     /label=RBS
     misc_feature    965..975
                     /label=BBa_B0033
     RBS             965..975
                     /label=BBa_B0033
     CDS             982..1584
                     /label=LasI
     misc_feature    1585..1617
                     /label=LVA
     misc_feature    1624..1648
                     /label=Help:Barcodes
     CDS             982..1623
                     /label=BBa_C0078
     stem_loop       1668..1711
                     /label=stem_loop
     misc_feature    1657..1736
                     /label=BBa_B0010
     terminator      1657..1736
                     /label=BBa_B0010
     stem_loop       1752..1771
                     /label=T7 TE
     misc_feature    1778..1778
                     /label=stop
     polyA_site      1772..1785
                     /label=polya
     misc_feature    1745..1785
                     /label=BBa_B0012
     terminator      1745..1785
                     /label=BBa_B0012
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtaca ctacctctgg cggtgatata ctagagaaag
      181 aggagaaata ctagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      241 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      301 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      361 tgccctggcc caccctcgtg accaccttcg gctacggcct gcaatgcttc gcccgctacc
      421 ccgaccacat gaagctgcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      481 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      541 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      601 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
      661 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
      721 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
      781 tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc
      841 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
      901 agctgtacaa gaggcctgct gcaaacgacg aaaactacgc tttagtagct taataatact
      961 agagtcacac aggactacta gatgatcgtt cagatcggtc gtcgtgaaga gttcgacaaa
     1021 aaactgctgg gtgaaatgca caaactgcgt gctcaggttt tcaaagaacg taaaggttgg
     1081 gacgtttccg ttatcgacga aatggaaatc gacggttacg acgctctgtc cccgtactac
     1141 atgctgatcc aggaagacac cccggaagct caggttttcg gttgctggcg tatcttcgac
     1201 accaccggtc cgtacatgct gaaaaacacc ttcccggaac tgctgcacgg taaagaagct
     1261 ccgtgctccc cgcacatctg ggaactgtcc cgtttcgcta tcaactccgg tcagaaaggt
     1321 tccctgggtt tctccgactg caccctggaa gctatgcgtg ctctggctcg ttactccttg
     1381 cagaacgaca tccagaccct ggttaccgtt accaccgttg gtgttgaaaa aatgatgatc
     1441 cgtgctggtc tggacgtttc ccgtttcggt ccgcacctga aaatcggtat cgaacgtgct
     1501 gttgctctgc gtatcgaact gaacgctaaa acccagatcg ctctgtacgg tggtgttctg
     1561 gttgaacagc gtctggctgt ttccgctgct aacgacgaaa actacgctct ggttgcttaa
     1621 taactctgat agtgctagtg tagatctcta ctagagccag gcatcaaata aaacgaaagg
     1681 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1741 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//