LOCUS       BBa_J06142               829 bp    DNA     linear       10-May-2024
ACCESSION   BBa_J06142
VERSION     BBa_J06142.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..77
                     /label=RBS-1\Strong
     RBS             70..74
                     /label=RBS
     misc_feature    63..77
                     /label=BBa_B0030
     RBS             63..77
                     /label=BBa_B0030
     CDS             84..692
                     /label=lasI
     CDS             84..692
                     /label=BBa_C0178
     stem_loop       712..755
                     /label=stem_loop
     misc_feature    701..780
                     /label=BBa_B0010
     terminator      701..780
                     /label=BBa_B0010
     stem_loop       796..815
                     /label=T7 TE
     misc_feature    822..822
                     /label=stop
     polyA_site      816..829
                     /label=polya
     misc_feature    789..829
                     /label=BBa_B0012
     terminator      789..829
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agattaaaga ggagaaatac tagatgatcg ttcagatcgg tcgtcgtgaa gagttcgaca
      121 aaaaactgct gggtgaaatg cacaaactgc gtgctcaggt tttcaaagaa cgtaaaggtt
      181 gggacgtttc cgttatcgac gaaatggaaa tcgacggtta cgacgctctg tccccgtact
      241 acatgctgat ccaggaagac accccggaag ctcaggtttt cggttgctgg cgtatcttcg
      301 acaccaccgg tccgtacatg ctgaaaaaca ccttcccgga actgctgcac ggtaaagaag
      361 ctccgtgctc cccgcacatc tgggaactgt cccgtttcgc tatcaactcc ggtcagaaag
      421 gttccctggg tttctccgac tgcaccctgg aagctatgcg tgctctggct cgttactcct
      481 tgcagaacga catccagacc ctggttaccg ttaccaccgt tggtgttgaa aaaatgatga
      541 tccgtgctgg tctggacgtt tcccgtttcg gtccgcacct gaaaatcggt atcgaacgtg
      601 ctgttgctct gcgtatcgaa ctgaacgcta aaacccagat cgctctgtac ggtggtgttc
      661 tggttgaaca gcgtctggct gtttcctaat aatactagag ccaggcatca aataaaacga
      721 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
      781 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//