LOCUS       BBa_J06144              2085 bp    DNA     linear       11-May-2024
ACCESSION   BBa_J06144
VERSION     BBa_J06144.1  
FEATURES             Location/Qualifiers
     promoter        4..9
                     /label=-35
     promoter        27..32
                     /label=-10
     misc_feature    1..38
                     /label=P(Cat)
     promoter        1..38
                     /label=BBa_I14033
     misc_feature    51..54
                     /label=conserved
     RBS             47..58
                     /label=BBa_B0034
     CDS             65..781
                     /label=lasR
     misc_feature    782..814
                     /label=LVA
     misc_feature    544..544
                     /label=G
     misc_feature    821..845
                     /label=Help:Barcodes
     CDS             65..820
                     /label=BBa_C0079
     stem_loop       865..908
                     /label=stem_loop
     misc_feature    854..933
                     /label=BBa_B0010
     terminator      854..933
                     /label=BBa_B0010
     stem_loop       949..968
                     /label=T7 TE
     misc_feature    975..975
                     /label=stop
     polyA_site      969..982
                     /label=polya
     misc_feature    942..982
                     /label=BBa_B0012
     terminator      942..982
                     /label=BBa_B0012
     misc_feature    1036..1053
                     /label=OP2
     misc_feature    1096..1115
                     /label=OP1
     promoter        1107..1112
                     /label=-35
     promoter        1130..1135
                     /label=-10
     promoter        991..1147
                     /label=BBa_R0079
     misc_feature    1160..1163
                     /label=conserved
     RBS             1156..1167
                     /label=BBa_B0034
     CDS             1177..1884
                     /label=cI lambda
     misc_feature    1885..1917
                     /label=LVA
     misc_feature    1924..1948
                     /label=Help:Barcodes
     CDS             1174..1923
                     /label=BBa_C0051
     stem_loop       1968..2011
                     /label=stem_loop
     misc_feature    1957..2036
                     /label=BBa_B0010
     terminator      1957..2036
                     /label=BBa_B0010
     stem_loop       2052..2071
                     /label=T7 TE
     misc_feature    2078..2078
                     /label=stop
     polyA_site      2072..2085
                     /label=polya
     misc_feature    2045..2085
                     /label=BBa_B0012
     terminator      2045..2085
                     /label=BBa_B0012
ORIGIN
        1 ggcacgtaag aggttccaac tttcaccata atgaaacata ctagagaaag aggagaaata
       61 ctagatggcc ttggttgacg gttttcttga gctggaacgc tcaagtggaa aattggagtg
      121 gagcgccatc ctccagaaga tggcgagcga ccttggattc tcgaagatcc tgttcggcct
      181 gttgcctaag gacagccagg actacgagaa cgccttcatc gtcggcaact acccggccgc
      241 ctggcgcgag cattacgacc gggctggcta cgcgcgggtc gacccgacgg tcagtcactg
      301 tacccagagc gtactgccga ttttctggga accgtccatc taccagacgc gaaagcagca
      361 cgagttcttc gaggaagcct cggccgccgg cctggtgtat gggctgacca tgccgctgca
      421 tggtgctcgc ggcgaactcg gcgcgctgag cctcagcgtg gaagcggaaa accgggccga
      481 ggccaaccgt ttcatagagt cggtcctgcc gaccctgtgg atgctcaagg actacgcact
      541 gcaaagcggt gccggactgg ccttcgaaca tccggtcagc aaaccggtgg ttctgaccag
      601 ccgggagaag gaagtgttgc agtggtgcgc catcggcaag accagttggg agatatcggt
      661 tatctgcaac tgctcggaag ccaatgtgaa cttccatatg ggaaatattc ggcggaagtt
      721 cggtgtgacc tcccgccgcg tagcggccat tatggccgtt aatttgggtc ttattactct
      781 cgctgcaaac gacgaaaact acgctttagt agcttaataa cactgatagt gctagtgtag
      841 atcactacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
      901 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
      961 ggtgggcctt tctgcgttta tatactagag gcccctcgct gagcgcgtcc cggagctggg
     1021 ggcaacctag ctgccacctg cttttctgct agctattcca gcgaaaacat acagatttcc
     1081 ggcgaaatca aggctacctg ccagttctgg caggtttggc cgcgggttct ttttggtaca
     1141 cgaaagctac tagagaaaga ggagaaatac tagatgagca caaaaaagaa accattaaca
     1201 caagagcagc ttgaggacgc acgtcgcctt aaagcaattt atgaaaaaaa gaaaaatgaa
     1261 cttggcttat cccaggaatc tgtcgcagac aagatgggga tggggcagtc aggcgttggt
     1321 gctttattta atggcatcaa tgcattaaat gcttataacg ccgcattgct tgcaaaaatt
     1381 ctcaaagtta gcgttgaaga atttagccct tcaatcgcca gagaaatcta cgagatgtat
     1441 gaagcggtta gtatgcagcc gtcacttaga agtgagtatg agtaccctgt tttttctcat
     1501 gttcaggcag ggatgttctc acctgagctt agaaccttta ccaaaggtga tgcggagaga
     1561 tgggtaagca caaccaaaaa agccagtgat tctgcattct ggcttgaggt tgaaggtaat
     1621 tccatgaccg caccaacagg ctccaagcca agctttcctg acggaatgtt aattctcgtt
     1681 gaccctgagc aggctgttga gccaggtgat ttctgcatag ccagacttgg gggtgatgag
     1741 tttaccttca agaaactgat cagggatagc ggtcaggtgt ttttacaacc actaaaccca
     1801 cagtacccaa tgatcccatg caatgagagt tgttccgttg tggggaaagt tatcgctagt
     1861 cagtggcctg aagagacgtt tggcgctgca aacgacgaaa actacgcttt agtagcttaa
     1921 taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg
     1981 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     2041 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//