LOCUS       BBa_J06145              1096 bp    DNA     linear       11-May-2024
ACCESSION   BBa_J06145
VERSION     BBa_J06145.1  
FEATURES             Location/Qualifiers
     misc_feature    1..151
                     /label=R0079
     misc_feature    152..168
                     /label=CI lambda O1
     promoter        117..122
                     /label=-35
     promoter        140..145
                     /label=-10
     promoter        1..168
                     /label=BBa_I14016
     RBS             177..191
                     /label=RBS-1\Strong
     RBS             184..188
                     /label=RBS
     misc_feature    177..191
                     /label=BBa_B0030
     RBS             177..191
                     /label=BBa_B0030
     CDS             198..959
                     /label=YFP (LVA)
     misc_feature    954..959
                     /label=2
     misc_feature    915..953
                     /label=SsrA
     misc_feature    439..439
                     /label=A (Q->L)
     misc_feature    198..959
                     /label=BBa_E0032
     misc_feature    198..959
                     /label=BBa_E0032
     stem_loop       979..1022
                     /label=stem_loop
     misc_feature    968..1047
                     /label=BBa_B0010
     terminator      968..1047
                     /label=BBa_B0010
     stem_loop       1063..1082
                     /label=T7 TE
     misc_feature    1089..1089
                     /label=stop
     polyA_site      1083..1096
                     /label=polya
     misc_feature    1056..1096
                     /label=BBa_B0012
     terminator      1056..1096
                     /label=BBa_B0012
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtaca ctacctctgg cggtgatata ctagagatta
      181 aagaggagaa atactagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      241 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      301 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      361 ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct
      421 accccgacca catgaagctg cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      481 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      541 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      601 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
      661 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
      721 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
      781 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga
      841 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
      901 acgagctgta caagaggcct gctgcaaacg acgaaaacta cgctttagta gcttaataat
      961 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     1021 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     1081 cctttctgcg tttata
//