LOCUS       BBa_J06147              1792 bp    DNA     linear       10-May-2024
ACCESSION   BBa_J06147
VERSION     BBa_J06147.1  
FEATURES             Location/Qualifiers
     misc_feature    1..151
                     /label=R0079
     misc_feature    152..168
                     /label=CI lambda O1
     promoter        117..122
                     /label=-35
     promoter        140..145
                     /label=-10
     promoter        1..168
                     /label=BBa_I14016
     RBS             177..191
                     /label=RBS-1\Strong
     RBS             184..188
                     /label=RBS
     misc_feature    177..191
                     /label=BBa_B0030
     RBS             177..191
                     /label=BBa_B0030
     CDS             198..959
                     /label=YFP (LVA)
     misc_feature    954..959
                     /label=2
     misc_feature    915..953
                     /label=SsrA
     misc_feature    439..439
                     /label=A (Q->L)
     misc_feature    198..959
                     /label=BBa_E0032
     misc_feature    198..959
                     /label=BBa_E0032
     RBS             968..982
                     /label=RBS-1\Strong
     RBS             975..979
                     /label=RBS
     misc_feature    968..982
                     /label=BBa_B0030
     RBS             968..982
                     /label=BBa_B0030
     CDS             989..1591
                     /label=LasI
     misc_feature    1592..1624
                     /label=LVA
     misc_feature    1631..1655
                     /label=Help:Barcodes
     CDS             989..1630
                     /label=BBa_C0078
     stem_loop       1675..1718
                     /label=stem_loop
     misc_feature    1664..1743
                     /label=BBa_B0010
     terminator      1664..1743
                     /label=BBa_B0010
     stem_loop       1759..1778
                     /label=T7 TE
     misc_feature    1785..1785
                     /label=stop
     polyA_site      1779..1792
                     /label=polya
     misc_feature    1752..1792
                     /label=BBa_B0012
     terminator      1752..1792
                     /label=BBa_B0012
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtaca ctacctctgg cggtgatata ctagagatta
      181 aagaggagaa atactagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      241 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      301 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      361 ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct
      421 accccgacca catgaagctg cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      481 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      541 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      601 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
      661 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
      721 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
      781 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga
      841 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
      901 acgagctgta caagaggcct gctgcaaacg acgaaaacta cgctttagta gcttaataat
      961 actagagatt aaagaggaga aatactagat gatcgttcag atcggtcgtc gtgaagagtt
     1021 cgacaaaaaa ctgctgggtg aaatgcacaa actgcgtgct caggttttca aagaacgtaa
     1081 aggttgggac gtttccgtta tcgacgaaat ggaaatcgac ggttacgacg ctctgtcccc
     1141 gtactacatg ctgatccagg aagacacccc ggaagctcag gttttcggtt gctggcgtat
     1201 cttcgacacc accggtccgt acatgctgaa aaacaccttc ccggaactgc tgcacggtaa
     1261 agaagctccg tgctccccgc acatctggga actgtcccgt ttcgctatca actccggtca
     1321 gaaaggttcc ctgggtttct ccgactgcac cctggaagct atgcgtgctc tggctcgtta
     1381 ctccttgcag aacgacatcc agaccctggt taccgttacc accgttggtg ttgaaaaaat
     1441 gatgatccgt gctggtctgg acgtttcccg tttcggtccg cacctgaaaa tcggtatcga
     1501 acgtgctgtt gctctgcgta tcgaactgaa cgctaaaacc cagatcgctc tgtacggtgg
     1561 tgttctggtt gaacagcgtc tggctgtttc cgctgctaac gacgaaaact acgctctggt
     1621 tgcttaataa ctctgatagt gctagtgtag atctctacta gagccaggca tcaaataaaa
     1681 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1741 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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