LOCUS       BBa_J06200              1969 bp    DNA     linear       10-May-2024
DEFINITION  C4HSL to YFP receiver test construct
ACCESSION   BBa_J06200
VERSION     BBa_J06200.1  
FEATURES             Location/Qualifiers
     misc_feature    1..35
                     /label=P(Bla)
     promoter        25..30
                     /label=-10
     promoter        4..9
                     /label=-35
     promoter        1..35
                     /label=BBa_I14018
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..784
                     /label=rhlR
     misc_feature    785..817
                     /label=LVA
     misc_feature    824..848
                     /label=Help:Barcodes
     CDS             62..823
                     /label=BBa_C0071
     stem_loop       868..911
                     /label=stem_loop
     misc_feature    857..936
                     /label=BBa_B0010
     terminator      857..936
                     /label=BBa_B0010
     stem_loop       952..971
                     /label=T7 TE
     misc_feature    978..978
                     /label=stop
     polyA_site      972..985
                     /label=polya
     misc_feature    945..985
                     /label=BBa_B0012
     terminator      945..985
                     /label=BBa_B0012
     misc_feature    994..1013
                     /label=RhlR
     misc_feature    994..1044
                     /label=modified P(Rhl)
     promoter        1014..1043
                     /label=-35
     promoter        994..1044
                     /label=BBa_I14017
     misc_feature    1057..1060
                     /label=conserved
     RBS             1053..1064
                     /label=BBa_B0034
     CDS             1071..1832
                     /label=YFP (LVA)
     misc_feature    1827..1832
                     /label=2
     misc_feature    1788..1826
                     /label=SsrA
     misc_feature    1312..1312
                     /label=A (Q->L)
     misc_feature    1071..1832
                     /label=BBa_E0032
     misc_feature    1071..1832
                     /label=BBa_E0032
     stem_loop       1852..1895
                     /label=stem_loop
     misc_feature    1841..1920
                     /label=BBa_B0010
     terminator      1841..1920
                     /label=BBa_B0010
     stem_loop       1936..1955
                     /label=T7 TE
     misc_feature    1962..1962
                     /label=stop
     polyA_site      1956..1969
                     /label=polya
     misc_feature    1929..1969
                     /label=BBa_B0012
     terminator      1929..1969
                     /label=BBa_B0012
ORIGIN
        1 tgtaagttta tacataggcg agtactctgt tatggtacta gagaaagagg agaaatacta
       61 gatgaggaat gacggaggct ttttgctgtg gtgggacggt ttgcgtagcg agatgcagcc
      121 gatccacgac agccagggcg tgttcgccgt cctggaaaag gaagtgcggc gcctgggctt
      181 cgattactac gcctatggcg tgcgccacac gattcccttc acccggccga agaccgaggt
      241 ccatggcacc tatcccaagg cctggctgga gcgataccag atgcagaact acggggccgt
      301 ggatccggcg atcctcaacg gcctgcgctc ctcggaaatg gtggtctgga gcgacagcct
      361 gttcgaccag agccggatgc tctggaacga ggctcgcgat tggggcctct gtgtcggcgc
      421 gaccttgccg atccgcgcgc cgaacaattt gctcagcgtg ctttccgtgg cgcgcgacca
      481 gcagaacatc tccagcttcg agcgcgagga aatccgcctg cggctgcgtt gcatgatcga
      541 gttgctgacc cagaagctga ccgacctgga gcatccgatg ctgatgtcca acccggtctg
      601 cctgagccat cgcgaacgcg agatcctgca atggaccgcc gacggcaaga gttccgggga
      661 aatcgccatc atcctgagca tctccgagag cacggtgaac ttccaccaca agaacatcca
      721 gaagaagttc gacgcgccga acaagacgct ggctgccgcc tacgccgcgg cgctgggtct
      781 catcgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat agtgctagtg
      841 tagatcacta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      901 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
      961 tcgggtgggc ctttctgcgt ttatatacta gagtcctgca tgaattggta ggcttacgca
     1021 agaaaatggt ttgttatagt cgaatactag agaaagagga gaaatactag atggtgagca
     1081 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     1141 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     1201 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1261 ccttcggcta cggcctgcaa tgcttcgccc gctaccccga ccacatgaag ctgcacgact
     1321 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     1381 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     1441 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1501 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     1561 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     1621 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct
     1681 accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     1741 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagagg cctgctgcaa
     1801 acgacgaaaa ctacgcttta gtagcttaat aatactagag ccaggcatca aataaaacga
     1861 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1921 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//