LOCUS       BBa_J06202               826 bp    DNA     linear       09-May-2024
DEFINITION  Tet repressible rhI generator (LVA-)
ACCESSION   BBa_J06202
VERSION     BBa_J06202.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..689
                     /label=rhII
     CDS             81..689
                     /label=BBa_C0170
     stem_loop       709..752
                     /label=stem_loop
     misc_feature    698..777
                     /label=BBa_B0010
     terminator      698..777
                     /label=BBa_B0010
     stem_loop       793..812
                     /label=T7 TE
     misc_feature    819..819
                     /label=stop
     polyA_site      813..826
                     /label=polya
     misc_feature    786..826
                     /label=BBa_B0012
     terminator      786..826
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgatcgaac tgctgtccga atccctggaa ggtctgtccg
      121 ctgctatgat cgctgaactg ggtcgttacc gtcaccaggt tttcatcgaa aaactgggtt
      181 gggacgttgt ttccacctcc cgtgttcgtg accaggagtt cgaccagttc gaccacccgc
      241 agacccgtta catcgttgct atgtcccgtc agggtatctg cggttgcgct cgtctgctgc
      301 cgaccaccga cgcttacctg ctgaaagacg ttttcgctta cctgtgctcc gaaaccccgc
      361 cgtccgaccc gtccgtttgg gaactgtccc gttacgctgc ttccgctgct gacgacccgc
      421 agctggctat gaaaatcttc tggtcctccc tccagtgcgc ttggtacctg ggtgcttcct
      481 ccgttgttgc tgttaccacc accgctatgg aacgttactt cgttcgtaac ggtgttatcc
      541 tccagcgtct gggtccgccg cagaaagtta aaggtgaaac cctggttgct atctccttcc
      601 cggcttacca ggaacgtggt ctggaaatgc tgctgcgtta ccacccggaa tggctccagg
      661 gtgttccgct gtccatggct gtttaataat actagagcca ggcatcaaat aaaacgaaag
      721 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      781 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//