LOCUS       BBa_J06212               828 bp    DNA     linear       12-May-2024
ACCESSION   BBa_J06212
VERSION     BBa_J06212.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    69..72
                     /label=conserved
     RBS             63..76
                     /label=BBa_B0031
     CDS             83..691
                     /label=rhII
     CDS             83..691
                     /label=BBa_C0170
     stem_loop       711..754
                     /label=stem_loop
     misc_feature    700..779
                     /label=BBa_B0010
     terminator      700..779
                     /label=BBa_B0010
     stem_loop       795..814
                     /label=T7 TE
     misc_feature    821..821
                     /label=stop
     polyA_site      815..828
                     /label=polya
     misc_feature    788..828
                     /label=BBa_B0012
     terminator      788..828
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaacctact agatgatcga actgctgtcc gaatccctgg aaggtctgtc
      121 cgctgctatg atcgctgaac tgggtcgtta ccgtcaccag gttttcatcg aaaaactggg
      181 ttgggacgtt gtttccacct cccgtgttcg tgaccaggag ttcgaccagt tcgaccaccc
      241 gcagacccgt tacatcgttg ctatgtcccg tcagggtatc tgcggttgcg ctcgtctgct
      301 gccgaccacc gacgcttacc tgctgaaaga cgttttcgct tacctgtgct ccgaaacccc
      361 gccgtccgac ccgtccgttt gggaactgtc ccgttacgct gcttccgctg ctgacgaccc
      421 gcagctggct atgaaaatct tctggtcctc cctccagtgc gcttggtacc tgggtgcttc
      481 ctccgttgtt gctgttacca ccaccgctat ggaacgttac ttcgttcgta acggtgttat
      541 cctccagcgt ctgggtccgc cgcagaaagt taaaggtgaa accctggttg ctatctcctt
      601 cccggcttac caggaacgtg gtctggaaat gctgctgcgt taccacccgg aatggctcca
      661 gggtgttccg ctgtccatgg ctgtttaata atactagagc caggcatcaa ataaaacgaa
      721 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
      781 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//