LOCUS       BBa_J06214              1984 bp    DNA     linear       10-May-2024
ACCESSION   BBa_J06214
VERSION     BBa_J06214.1  
FEATURES             Location/Qualifiers
     misc_feature    1..35
                     /label=P(Bla)
     promoter        25..30
                     /label=-10
     promoter        4..9
                     /label=-35
     promoter        1..35
                     /label=BBa_I14018
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..784
                     /label=rhlR
     misc_feature    785..817
                     /label=LVA
     misc_feature    824..848
                     /label=Help:Barcodes
     CDS             62..823
                     /label=BBa_C0071
     stem_loop       868..911
                     /label=stem_loop
     misc_feature    857..936
                     /label=BBa_B0010
     terminator      857..936
                     /label=BBa_B0010
     stem_loop       952..971
                     /label=T7 TE
     misc_feature    978..978
                     /label=stop
     polyA_site      972..985
                     /label=polya
     misc_feature    945..985
                     /label=BBa_B0012
     terminator      945..985
                     /label=BBa_B0012
     misc_feature    994..1013
                     /label=RhlR
     misc_feature    994..1044
                     /label=modified P(Rhl)
     promoter        1014..1043
                     /label=-35
     promoter        994..1044
                     /label=BBa_I14017
     misc_feature    1059..1062
                     /label=conserved
     RBS             1053..1066
                     /label=BBa_B0031
     CDS             1076..1783
                     /label=cI lambda
     misc_feature    1784..1816
                     /label=LVA
     misc_feature    1823..1847
                     /label=Help:Barcodes
     CDS             1073..1822
                     /label=BBa_C0051
     stem_loop       1867..1910
                     /label=stem_loop
     misc_feature    1856..1935
                     /label=BBa_B0010
     terminator      1856..1935
                     /label=BBa_B0010
     stem_loop       1951..1970
                     /label=T7 TE
     misc_feature    1977..1977
                     /label=stop
     polyA_site      1971..1984
                     /label=polya
     misc_feature    1944..1984
                     /label=BBa_B0012
     terminator      1944..1984
                     /label=BBa_B0012
ORIGIN
        1 tgtaagttta tacataggcg agtactctgt tatggtacta gagaaagagg agaaatacta
       61 gatgaggaat gacggaggct ttttgctgtg gtgggacggt ttgcgtagcg agatgcagcc
      121 gatccacgac agccagggcg tgttcgccgt cctggaaaag gaagtgcggc gcctgggctt
      181 cgattactac gcctatggcg tgcgccacac gattcccttc acccggccga agaccgaggt
      241 ccatggcacc tatcccaagg cctggctgga gcgataccag atgcagaact acggggccgt
      301 ggatccggcg atcctcaacg gcctgcgctc ctcggaaatg gtggtctgga gcgacagcct
      361 gttcgaccag agccggatgc tctggaacga ggctcgcgat tggggcctct gtgtcggcgc
      421 gaccttgccg atccgcgcgc cgaacaattt gctcagcgtg ctttccgtgg cgcgcgacca
      481 gcagaacatc tccagcttcg agcgcgagga aatccgcctg cggctgcgtt gcatgatcga
      541 gttgctgacc cagaagctga ccgacctgga gcatccgatg ctgatgtcca acccggtctg
      601 cctgagccat cgcgaacgcg agatcctgca atggaccgcc gacggcaaga gttccgggga
      661 aatcgccatc atcctgagca tctccgagag cacggtgaac ttccaccaca agaacatcca
      721 gaagaagttc gacgcgccga acaagacgct ggctgccgcc tacgccgcgg cgctgggtct
      781 catcgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat agtgctagtg
      841 tagatcacta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      901 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
      961 tcgggtgggc ctttctgcgt ttatatacta gagtcctgca tgaattggta ggcttacgca
     1021 agaaaatggt ttgttatagt cgaatactag agtcacacag gaaacctact agatgagcac
     1081 aaaaaagaaa ccattaacac aagagcagct tgaggacgca cgtcgcctta aagcaattta
     1141 tgaaaaaaag aaaaatgaac ttggcttatc ccaggaatct gtcgcagaca agatggggat
     1201 ggggcagtca ggcgttggtg ctttatttaa tggcatcaat gcattaaatg cttataacgc
     1261 cgcattgctt gcaaaaattc tcaaagttag cgttgaagaa tttagccctt caatcgccag
     1321 agaaatctac gagatgtatg aagcggttag tatgcagccg tcacttagaa gtgagtatga
     1381 gtaccctgtt ttttctcatg ttcaggcagg gatgttctca cctgagctta gaacctttac
     1441 caaaggtgat gcggagagat gggtaagcac aaccaaaaaa gccagtgatt ctgcattctg
     1501 gcttgaggtt gaaggtaatt ccatgaccgc accaacaggc tccaagccaa gctttcctga
     1561 cggaatgtta attctcgttg accctgagca ggctgttgag ccaggtgatt tctgcatagc
     1621 cagacttggg ggtgatgagt ttaccttcaa gaaactgatc agggatagcg gtcaggtgtt
     1681 tttacaacca ctaaacccac agtacccaat gatcccatgc aatgagagtt gttccgttgt
     1741 ggggaaagtt atcgctagtc agtggcctga agagacgttt ggcgctgcaa acgacgaaaa
     1801 ctacgcttta gtagcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg
     1861 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1921 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     1981 tata
//