LOCUS       BBa_J06218              1613 bp    DNA     linear       17-May-2024
ACCESSION   BBa_J06218
VERSION     BBa_J06218.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=RhlR
     misc_feature    1..51
                     /label=modified P(Rhl)
     promoter        21..50
                     /label=-35
     promoter        1..51
                     /label=BBa_I14017
     misc_feature    64..67
                     /label=conserved
     RBS             60..71
                     /label=BBa_B0034
     CDS             78..839
                     /label=YFP (LVA)
     misc_feature    834..839
                     /label=2
     misc_feature    795..833
                     /label=SsrA
     misc_feature    319..319
                     /label=A (Q->L)
     misc_feature    78..839
                     /label=BBa_E0032
     misc_feature    78..839
                     /label=BBa_E0032
     misc_feature    854..857
                     /label=conserved
     RBS             848..861
                     /label=BBa_B0031
     CDS             868..1476
                     /label=rhII
     CDS             868..1476
                     /label=BBa_C0170
     stem_loop       1496..1539
                     /label=stem_loop
     misc_feature    1485..1564
                     /label=BBa_B0010
     terminator      1485..1564
                     /label=BBa_B0010
     stem_loop       1580..1599
                     /label=T7 TE
     misc_feature    1606..1606
                     /label=stop
     polyA_site      1600..1613
                     /label=polya
     misc_feature    1573..1613
                     /label=BBa_B0012
     terminator      1573..1613
                     /label=BBa_B0012
ORIGIN
        1 tcctgcatga attggtaggc ttacgcaaga aaatggtttg ttatagtcga atactagaga
       61 aagaggagaa atactagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      121 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      181 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      241 ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct
      301 accccgacca catgaagctg cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      361 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      421 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      481 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
      541 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
      601 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
      661 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga
      721 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
      781 acgagctgta caagaggcct gctgcaaacg acgaaaacta cgctttagta gcttaataat
      841 actagagtca cacaggaaac ctactagatg atcgaactgc tgtccgaatc cctggaaggt
      901 ctgtccgctg ctatgatcgc tgaactgggt cgttaccgtc accaggtttt catcgaaaaa
      961 ctgggttggg acgttgtttc cacctcccgt gttcgtgacc aggagttcga ccagttcgac
     1021 cacccgcaga cccgttacat cgttgctatg tcccgtcagg gtatctgcgg ttgcgctcgt
     1081 ctgctgccga ccaccgacgc ttacctgctg aaagacgttt tcgcttacct gtgctccgaa
     1141 accccgccgt ccgacccgtc cgtttgggaa ctgtcccgtt acgctgcttc cgctgctgac
     1201 gacccgcagc tggctatgaa aatcttctgg tcctccctcc agtgcgcttg gtacctgggt
     1261 gcttcctccg ttgttgctgt taccaccacc gctatggaac gttacttcgt tcgtaacggt
     1321 gttatcctcc agcgtctggg tccgccgcag aaagttaaag gtgaaaccct ggttgctatc
     1381 tccttcccgg cttaccagga acgtggtctg gaaatgctgc tgcgttacca cccggaatgg
     1441 ctccagggtg ttccgctgtc catggctgtt taataatact agagccaggc atcaaataaa
     1501 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     1561 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//