LOCUS       BBa_J06222               827 bp    DNA     linear       13-May-2024
ACCESSION   BBa_J06222
VERSION     BBa_J06222.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..75
                     /label=RBS-3\Weak
     RBS             69..72
                     /label=RBS
     misc_feature    63..75
                     /label=BBa_B0032
     RBS             63..75
                     /label=BBa_B0032
     CDS             82..690
                     /label=rhII
     CDS             82..690
                     /label=BBa_C0170
     stem_loop       710..753
                     /label=stem_loop
     misc_feature    699..778
                     /label=BBa_B0010
     terminator      699..778
                     /label=BBa_B0010
     stem_loop       794..813
                     /label=T7 TE
     misc_feature    820..820
                     /label=stop
     polyA_site      814..827
                     /label=polya
     misc_feature    787..827
                     /label=BBa_B0012
     terminator      787..827
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaagtacta gatgatcgaa ctgctgtccg aatccctgga aggtctgtcc
      121 gctgctatga tcgctgaact gggtcgttac cgtcaccagg ttttcatcga aaaactgggt
      181 tgggacgttg tttccacctc ccgtgttcgt gaccaggagt tcgaccagtt cgaccacccg
      241 cagacccgtt acatcgttgc tatgtcccgt cagggtatct gcggttgcgc tcgtctgctg
      301 ccgaccaccg acgcttacct gctgaaagac gttttcgctt acctgtgctc cgaaaccccg
      361 ccgtccgacc cgtccgtttg ggaactgtcc cgttacgctg cttccgctgc tgacgacccg
      421 cagctggcta tgaaaatctt ctggtcctcc ctccagtgcg cttggtacct gggtgcttcc
      481 tccgttgttg ctgttaccac caccgctatg gaacgttact tcgttcgtaa cggtgttatc
      541 ctccagcgtc tgggtccgcc gcagaaagtt aaaggtgaaa ccctggttgc tatctccttc
      601 ccggcttacc aggaacgtgg tctggaaatg ctgctgcgtt accacccgga atggctccag
      661 ggtgttccgc tgtccatggc tgtttaataa tactagagcc aggcatcaaa taaaacgaaa
      721 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
      781 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//