LOCUS       BBa_J06224              1983 bp    DNA     linear       09-May-2024
ACCESSION   BBa_J06224
VERSION     BBa_J06224.1  
FEATURES             Location/Qualifiers
     misc_feature    1..35
                     /label=P(Bla)
     promoter        25..30
                     /label=-10
     promoter        4..9
                     /label=-35
     promoter        1..35
                     /label=BBa_I14018
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..784
                     /label=rhlR
     misc_feature    785..817
                     /label=LVA
     misc_feature    824..848
                     /label=Help:Barcodes
     CDS             62..823
                     /label=BBa_C0071
     stem_loop       868..911
                     /label=stem_loop
     misc_feature    857..936
                     /label=BBa_B0010
     terminator      857..936
                     /label=BBa_B0010
     stem_loop       952..971
                     /label=T7 TE
     misc_feature    978..978
                     /label=stop
     polyA_site      972..985
                     /label=polya
     misc_feature    945..985
                     /label=BBa_B0012
     terminator      945..985
                     /label=BBa_B0012
     misc_feature    994..1013
                     /label=RhlR
     misc_feature    994..1044
                     /label=modified P(Rhl)
     promoter        1014..1043
                     /label=-35
     promoter        994..1044
                     /label=BBa_I14017
     RBS             1053..1065
                     /label=RBS-3\Weak
     RBS             1059..1062
                     /label=RBS
     misc_feature    1053..1065
                     /label=BBa_B0032
     RBS             1053..1065
                     /label=BBa_B0032
     CDS             1075..1782
                     /label=cI lambda
     misc_feature    1783..1815
                     /label=LVA
     misc_feature    1822..1846
                     /label=Help:Barcodes
     CDS             1072..1821
                     /label=BBa_C0051
     stem_loop       1866..1909
                     /label=stem_loop
     misc_feature    1855..1934
                     /label=BBa_B0010
     terminator      1855..1934
                     /label=BBa_B0010
     stem_loop       1950..1969
                     /label=T7 TE
     misc_feature    1976..1976
                     /label=stop
     polyA_site      1970..1983
                     /label=polya
     misc_feature    1943..1983
                     /label=BBa_B0012
     terminator      1943..1983
                     /label=BBa_B0012
ORIGIN
        1 tgtaagttta tacataggcg agtactctgt tatggtacta gagaaagagg agaaatacta
       61 gatgaggaat gacggaggct ttttgctgtg gtgggacggt ttgcgtagcg agatgcagcc
      121 gatccacgac agccagggcg tgttcgccgt cctggaaaag gaagtgcggc gcctgggctt
      181 cgattactac gcctatggcg tgcgccacac gattcccttc acccggccga agaccgaggt
      241 ccatggcacc tatcccaagg cctggctgga gcgataccag atgcagaact acggggccgt
      301 ggatccggcg atcctcaacg gcctgcgctc ctcggaaatg gtggtctgga gcgacagcct
      361 gttcgaccag agccggatgc tctggaacga ggctcgcgat tggggcctct gtgtcggcgc
      421 gaccttgccg atccgcgcgc cgaacaattt gctcagcgtg ctttccgtgg cgcgcgacca
      481 gcagaacatc tccagcttcg agcgcgagga aatccgcctg cggctgcgtt gcatgatcga
      541 gttgctgacc cagaagctga ccgacctgga gcatccgatg ctgatgtcca acccggtctg
      601 cctgagccat cgcgaacgcg agatcctgca atggaccgcc gacggcaaga gttccgggga
      661 aatcgccatc atcctgagca tctccgagag cacggtgaac ttccaccaca agaacatcca
      721 gaagaagttc gacgcgccga acaagacgct ggctgccgcc tacgccgcgg cgctgggtct
      781 catcgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat agtgctagtg
      841 tagatcacta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      901 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
      961 tcgggtgggc ctttctgcgt ttatatacta gagtcctgca tgaattggta ggcttacgca
     1021 agaaaatggt ttgttatagt cgaatactag agtcacacag gaaagtacta gatgagcaca
     1081 aaaaagaaac cattaacaca agagcagctt gaggacgcac gtcgccttaa agcaatttat
     1141 gaaaaaaaga aaaatgaact tggcttatcc caggaatctg tcgcagacaa gatggggatg
     1201 gggcagtcag gcgttggtgc tttatttaat ggcatcaatg cattaaatgc ttataacgcc
     1261 gcattgcttg caaaaattct caaagttagc gttgaagaat ttagcccttc aatcgccaga
     1321 gaaatctacg agatgtatga agcggttagt atgcagccgt cacttagaag tgagtatgag
     1381 taccctgttt tttctcatgt tcaggcaggg atgttctcac ctgagcttag aacctttacc
     1441 aaaggtgatg cggagagatg ggtaagcaca accaaaaaag ccagtgattc tgcattctgg
     1501 cttgaggttg aaggtaattc catgaccgca ccaacaggct ccaagccaag ctttcctgac
     1561 ggaatgttaa ttctcgttga ccctgagcag gctgttgagc caggtgattt ctgcatagcc
     1621 agacttgggg gtgatgagtt taccttcaag aaactgatca gggatagcgg tcaggtgttt
     1681 ttacaaccac taaacccaca gtacccaatg atcccatgca atgagagttg ttccgttgtg
     1741 gggaaagtta tcgctagtca gtggcctgaa gagacgtttg gcgctgcaaa cgacgaaaac
     1801 tacgctttag tagcttaata acgctgatag tgctagtgta gatcgctact agagccaggc
     1861 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1921 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1981 ata
//