LOCUS       BBa_J06225               977 bp    DNA     linear       10-May-2024
ACCESSION   BBa_J06225
VERSION     BBa_J06225.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=RhlR
     misc_feature    1..51
                     /label=modified P(Rhl)
     promoter        21..50
                     /label=-35
     promoter        1..51
                     /label=BBa_I14017
     RBS             60..72
                     /label=RBS-3\Weak
     RBS             66..69
                     /label=RBS
     misc_feature    60..72
                     /label=BBa_B0032
     RBS             60..72
                     /label=BBa_B0032
     CDS             79..840
                     /label=YFP (LVA)
     misc_feature    835..840
                     /label=2
     misc_feature    796..834
                     /label=SsrA
     misc_feature    320..320
                     /label=A (Q->L)
     misc_feature    79..840
                     /label=BBa_E0032
     misc_feature    79..840
                     /label=BBa_E0032
     stem_loop       860..903
                     /label=stem_loop
     misc_feature    849..928
                     /label=BBa_B0010
     terminator      849..928
                     /label=BBa_B0010
     stem_loop       944..963
                     /label=T7 TE
     misc_feature    970..970
                     /label=stop
     polyA_site      964..977
                     /label=polya
     misc_feature    937..977
                     /label=BBa_B0012
     terminator      937..977
                     /label=BBa_B0012
ORIGIN
        1 tcctgcatga attggtaggc ttacgcaaga aaatggtttg ttatagtcga atactagagt
       61 cacacaggaa agtactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
      121 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
      181 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      241 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcaatg cttcgcccgc
      301 taccccgacc acatgaagct gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      361 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
      421 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
      481 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
      541 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
      601 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
      661 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag
      721 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
      781 gacgagctgt acaagaggcc tgctgcaaac gacgaaaact acgctttagt agcttaataa
      841 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      901 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      961 gcctttctgc gtttata
//