LOCUS       BBa_J06232               825 bp    DNA     linear       12-May-2024
ACCESSION   BBa_J06232
VERSION     BBa_J06232.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..73
                     /label=RBS-4\Weaker
     RBS             69..72
                     /label=RBS
     misc_feature    63..73
                     /label=BBa_B0033
     RBS             63..73
                     /label=BBa_B0033
     CDS             80..688
                     /label=rhII
     CDS             80..688
                     /label=BBa_C0170
     stem_loop       708..751
                     /label=stem_loop
     misc_feature    697..776
                     /label=BBa_B0010
     terminator      697..776
                     /label=BBa_B0010
     stem_loop       792..811
                     /label=T7 TE
     misc_feature    818..818
                     /label=stop
     polyA_site      812..825
                     /label=polya
     misc_feature    785..825
                     /label=BBa_B0012
     terminator      785..825
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gactactaga tgatcgaact gctgtccgaa tccctggaag gtctgtccgc
      121 tgctatgatc gctgaactgg gtcgttaccg tcaccaggtt ttcatcgaaa aactgggttg
      181 ggacgttgtt tccacctccc gtgttcgtga ccaggagttc gaccagttcg accacccgca
      241 gacccgttac atcgttgcta tgtcccgtca gggtatctgc ggttgcgctc gtctgctgcc
      301 gaccaccgac gcttacctgc tgaaagacgt tttcgcttac ctgtgctccg aaaccccgcc
      361 gtccgacccg tccgtttggg aactgtcccg ttacgctgct tccgctgctg acgacccgca
      421 gctggctatg aaaatcttct ggtcctccct ccagtgcgct tggtacctgg gtgcttcctc
      481 cgttgttgct gttaccacca ccgctatgga acgttacttc gttcgtaacg gtgttatcct
      541 ccagcgtctg ggtccgccgc agaaagttaa aggtgaaacc ctggttgcta tctccttccc
      601 ggcttaccag gaacgtggtc tggaaatgct gctgcgttac cacccggaat ggctccaggg
      661 tgttccgctg tccatggctg tttaataata ctagagccag gcatcaaata aaacgaaagg
      721 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
      781 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//