LOCUS       BBa_J06234              1981 bp    DNA     linear       10-May-2024
ACCESSION   BBa_J06234
VERSION     BBa_J06234.1  
FEATURES             Location/Qualifiers
     misc_feature    1..35
                     /label=P(Bla)
     promoter        25..30
                     /label=-10
     promoter        4..9
                     /label=-35
     promoter        1..35
                     /label=BBa_I14018
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..784
                     /label=rhlR
     misc_feature    785..817
                     /label=LVA
     misc_feature    824..848
                     /label=Help:Barcodes
     CDS             62..823
                     /label=BBa_C0071
     stem_loop       868..911
                     /label=stem_loop
     misc_feature    857..936
                     /label=BBa_B0010
     terminator      857..936
                     /label=BBa_B0010
     stem_loop       952..971
                     /label=T7 TE
     misc_feature    978..978
                     /label=stop
     polyA_site      972..985
                     /label=polya
     misc_feature    945..985
                     /label=BBa_B0012
     terminator      945..985
                     /label=BBa_B0012
     misc_feature    994..1013
                     /label=RhlR
     misc_feature    994..1044
                     /label=modified P(Rhl)
     promoter        1014..1043
                     /label=-35
     promoter        994..1044
                     /label=BBa_I14017
     RBS             1053..1063
                     /label=RBS-4\Weaker
     RBS             1059..1062
                     /label=RBS
     misc_feature    1053..1063
                     /label=BBa_B0033
     RBS             1053..1063
                     /label=BBa_B0033
     CDS             1073..1780
                     /label=cI lambda
     misc_feature    1781..1813
                     /label=LVA
     misc_feature    1820..1844
                     /label=Help:Barcodes
     CDS             1070..1819
                     /label=BBa_C0051
     stem_loop       1864..1907
                     /label=stem_loop
     misc_feature    1853..1932
                     /label=BBa_B0010
     terminator      1853..1932
                     /label=BBa_B0010
     stem_loop       1948..1967
                     /label=T7 TE
     misc_feature    1974..1974
                     /label=stop
     polyA_site      1968..1981
                     /label=polya
     misc_feature    1941..1981
                     /label=BBa_B0012
     terminator      1941..1981
                     /label=BBa_B0012
ORIGIN
        1 tgtaagttta tacataggcg agtactctgt tatggtacta gagaaagagg agaaatacta
       61 gatgaggaat gacggaggct ttttgctgtg gtgggacggt ttgcgtagcg agatgcagcc
      121 gatccacgac agccagggcg tgttcgccgt cctggaaaag gaagtgcggc gcctgggctt
      181 cgattactac gcctatggcg tgcgccacac gattcccttc acccggccga agaccgaggt
      241 ccatggcacc tatcccaagg cctggctgga gcgataccag atgcagaact acggggccgt
      301 ggatccggcg atcctcaacg gcctgcgctc ctcggaaatg gtggtctgga gcgacagcct
      361 gttcgaccag agccggatgc tctggaacga ggctcgcgat tggggcctct gtgtcggcgc
      421 gaccttgccg atccgcgcgc cgaacaattt gctcagcgtg ctttccgtgg cgcgcgacca
      481 gcagaacatc tccagcttcg agcgcgagga aatccgcctg cggctgcgtt gcatgatcga
      541 gttgctgacc cagaagctga ccgacctgga gcatccgatg ctgatgtcca acccggtctg
      601 cctgagccat cgcgaacgcg agatcctgca atggaccgcc gacggcaaga gttccgggga
      661 aatcgccatc atcctgagca tctccgagag cacggtgaac ttccaccaca agaacatcca
      721 gaagaagttc gacgcgccga acaagacgct ggctgccgcc tacgccgcgg cgctgggtct
      781 catcgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat agtgctagtg
      841 tagatcacta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      901 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
      961 tcgggtgggc ctttctgcgt ttatatacta gagtcctgca tgaattggta ggcttacgca
     1021 agaaaatggt ttgttatagt cgaatactag agtcacacag gactactaga tgagcacaaa
     1081 aaagaaacca ttaacacaag agcagcttga ggacgcacgt cgccttaaag caatttatga
     1141 aaaaaagaaa aatgaacttg gcttatccca ggaatctgtc gcagacaaga tggggatggg
     1201 gcagtcaggc gttggtgctt tatttaatgg catcaatgca ttaaatgctt ataacgccgc
     1261 attgcttgca aaaattctca aagttagcgt tgaagaattt agcccttcaa tcgccagaga
     1321 aatctacgag atgtatgaag cggttagtat gcagccgtca cttagaagtg agtatgagta
     1381 ccctgttttt tctcatgttc aggcagggat gttctcacct gagcttagaa cctttaccaa
     1441 aggtgatgcg gagagatggg taagcacaac caaaaaagcc agtgattctg cattctggct
     1501 tgaggttgaa ggtaattcca tgaccgcacc aacaggctcc aagccaagct ttcctgacgg
     1561 aatgttaatt ctcgttgacc ctgagcaggc tgttgagcca ggtgatttct gcatagccag
     1621 acttgggggt gatgagttta ccttcaagaa actgatcagg gatagcggtc aggtgttttt
     1681 acaaccacta aacccacagt acccaatgat cccatgcaat gagagttgtt ccgttgtggg
     1741 gaaagttatc gctagtcagt ggcctgaaga gacgtttggc gctgcaaacg acgaaaacta
     1801 cgctttagta gcttaataac gctgatagtg ctagtgtaga tcgctactag agccaggcat
     1861 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1921 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     1981 a
//