LOCUS       BBa_J06235               975 bp    DNA     linear       13-May-2024
ACCESSION   BBa_J06235
VERSION     BBa_J06235.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=RhlR
     misc_feature    1..51
                     /label=modified P(Rhl)
     promoter        21..50
                     /label=-35
     promoter        1..51
                     /label=BBa_I14017
     RBS             60..70
                     /label=RBS-4\Weaker
     RBS             66..69
                     /label=RBS
     misc_feature    60..70
                     /label=BBa_B0033
     RBS             60..70
                     /label=BBa_B0033
     CDS             77..838
                     /label=YFP (LVA)
     misc_feature    833..838
                     /label=2
     misc_feature    794..832
                     /label=SsrA
     misc_feature    318..318
                     /label=A (Q->L)
     misc_feature    77..838
                     /label=BBa_E0032
     misc_feature    77..838
                     /label=BBa_E0032
     stem_loop       858..901
                     /label=stem_loop
     misc_feature    847..926
                     /label=BBa_B0010
     terminator      847..926
                     /label=BBa_B0010
     stem_loop       942..961
                     /label=T7 TE
     misc_feature    968..968
                     /label=stop
     polyA_site      962..975
                     /label=polya
     misc_feature    935..975
                     /label=BBa_B0012
     terminator      935..975
                     /label=BBa_B0012
ORIGIN
        1 tcctgcatga attggtaggc ttacgcaaga aaatggtttg ttatagtcga atactagagt
       61 cacacaggac tactagatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
      121 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
      181 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
      241 cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgcaatgct tcgcccgcta
      301 ccccgaccac atgaagctgc acgacttctt caagtccgcc atgcccgaag gctacgtcca
      361 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
      421 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
      481 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
      541 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
      601 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
      661 gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa
      721 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
      781 cgagctgtac aagaggcctg ctgcaaacga cgaaaactac gctttagtag cttaataata
      841 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
      901 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
      961 ctttctgcgt ttata
//