LOCUS       BBa_J06238              1610 bp    DNA     linear       17-May-2024
ACCESSION   BBa_J06238
VERSION     BBa_J06238.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=RhlR
     misc_feature    1..51
                     /label=modified P(Rhl)
     promoter        21..50
                     /label=-35
     promoter        1..51
                     /label=BBa_I14017
     misc_feature    64..67
                     /label=conserved
     RBS             60..71
                     /label=BBa_B0034
     CDS             78..839
                     /label=YFP (LVA)
     misc_feature    834..839
                     /label=2
     misc_feature    795..833
                     /label=SsrA
     misc_feature    319..319
                     /label=A (Q->L)
     misc_feature    78..839
                     /label=BBa_E0032
     misc_feature    78..839
                     /label=BBa_E0032
     RBS             848..858
                     /label=RBS-4\Weaker
     RBS             854..857
                     /label=RBS
     misc_feature    848..858
                     /label=BBa_B0033
     RBS             848..858
                     /label=BBa_B0033
     CDS             865..1473
                     /label=rhII
     CDS             865..1473
                     /label=BBa_C0170
     stem_loop       1493..1536
                     /label=stem_loop
     misc_feature    1482..1561
                     /label=BBa_B0010
     terminator      1482..1561
                     /label=BBa_B0010
     stem_loop       1577..1596
                     /label=T7 TE
     misc_feature    1603..1603
                     /label=stop
     polyA_site      1597..1610
                     /label=polya
     misc_feature    1570..1610
                     /label=BBa_B0012
     terminator      1570..1610
                     /label=BBa_B0012
ORIGIN
        1 tcctgcatga attggtaggc ttacgcaaga aaatggtttg ttatagtcga atactagaga
       61 aagaggagaa atactagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      121 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      181 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      241 ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct
      301 accccgacca catgaagctg cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      361 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      421 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      481 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
      541 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
      601 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
      661 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga
      721 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
      781 acgagctgta caagaggcct gctgcaaacg acgaaaacta cgctttagta gcttaataat
      841 actagagtca cacaggacta ctagatgatc gaactgctgt ccgaatccct ggaaggtctg
      901 tccgctgcta tgatcgctga actgggtcgt taccgtcacc aggttttcat cgaaaaactg
      961 ggttgggacg ttgtttccac ctcccgtgtt cgtgaccagg agttcgacca gttcgaccac
     1021 ccgcagaccc gttacatcgt tgctatgtcc cgtcagggta tctgcggttg cgctcgtctg
     1081 ctgccgacca ccgacgctta cctgctgaaa gacgttttcg cttacctgtg ctccgaaacc
     1141 ccgccgtccg acccgtccgt ttgggaactg tcccgttacg ctgcttccgc tgctgacgac
     1201 ccgcagctgg ctatgaaaat cttctggtcc tccctccagt gcgcttggta cctgggtgct
     1261 tcctccgttg ttgctgttac caccaccgct atggaacgtt acttcgttcg taacggtgtt
     1321 atcctccagc gtctgggtcc gccgcagaaa gttaaaggtg aaaccctggt tgctatctcc
     1381 ttcccggctt accaggaacg tggtctggaa atgctgctgc gttaccaccc ggaatggctc
     1441 cagggtgttc cgctgtccat ggctgtttaa taatactaga gccaggcatc aaataaaacg
     1501 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1561 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//