LOCUS       BBa_J06242               829 bp    DNA     linear       10-May-2024
ACCESSION   BBa_J06242
VERSION     BBa_J06242.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..77
                     /label=RBS-1\Strong
     RBS             70..74
                     /label=RBS
     misc_feature    63..77
                     /label=BBa_B0030
     RBS             63..77
                     /label=BBa_B0030
     CDS             84..692
                     /label=rhII
     CDS             84..692
                     /label=BBa_C0170
     stem_loop       712..755
                     /label=stem_loop
     misc_feature    701..780
                     /label=BBa_B0010
     terminator      701..780
                     /label=BBa_B0010
     stem_loop       796..815
                     /label=T7 TE
     misc_feature    822..822
                     /label=stop
     polyA_site      816..829
                     /label=polya
     misc_feature    789..829
                     /label=BBa_B0012
     terminator      789..829
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agattaaaga ggagaaatac tagatgatcg aactgctgtc cgaatccctg gaaggtctgt
      121 ccgctgctat gatcgctgaa ctgggtcgtt accgtcacca ggttttcatc gaaaaactgg
      181 gttgggacgt tgtttccacc tcccgtgttc gtgaccagga gttcgaccag ttcgaccacc
      241 cgcagacccg ttacatcgtt gctatgtccc gtcagggtat ctgcggttgc gctcgtctgc
      301 tgccgaccac cgacgcttac ctgctgaaag acgttttcgc ttacctgtgc tccgaaaccc
      361 cgccgtccga cccgtccgtt tgggaactgt cccgttacgc tgcttccgct gctgacgacc
      421 cgcagctggc tatgaaaatc ttctggtcct ccctccagtg cgcttggtac ctgggtgctt
      481 cctccgttgt tgctgttacc accaccgcta tggaacgtta cttcgttcgt aacggtgtta
      541 tcctccagcg tctgggtccg ccgcagaaag ttaaaggtga aaccctggtt gctatctcct
      601 tcccggctta ccaggaacgt ggtctggaaa tgctgctgcg ttaccacccg gaatggctcc
      661 agggtgttcc gctgtccatg gctgtttaat aatactagag ccaggcatca aataaaacga
      721 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
      781 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//