LOCUS       BBa_J06451              1653 bp    DNA     linear       29-May-2024
ACCESSION   BBa_J06451
VERSION     BBa_J06451.1  
FEATURES             Location/Qualifiers
     misc_feature    1..97
                     /label=BBa_R0065
     misc_feature    6..25
                     /label=Lux Box
     promoter        47..52
                     /label=-10
     misc_feature    57..73
                     /label=OR2 cI
     promoter        58..58
                     /label=lux p(R) start
     promoter        71..76
                     /label=-35
     misc_feature    81..97
                     /label=OR1 cI
     promoter        94..97
                     /label=-10
     promoter        1..97
                     /label=BBa_R0065
     misc_feature    110..113
                     /label=conserved
     RBS             106..117
                     /label=BBa_B0034
     CDS             124..846
                     /label=BBa_E0030
     RBS             855..867
                     /label=RBS-3\Weak
     RBS             861..864
                     /label=RBS
     misc_feature    855..867
                     /label=BBa_B0032
     RBS             855..867
                     /label=BBa_B0032
     misc_feature    1453..1484
                     /label=LVA
     CDS             874..1452
                     /label=luxI
     misc_feature    874..1491
                     /label=BBa_C0061
     misc_feature    1492..1516
                     /label=Help:Barcodes
     CDS             874..1491
                     /label=BBa_C0061
     stem_loop       1536..1579
                     /label=stem_loop
     misc_feature    1525..1604
                     /label=BBa_B0010
     terminator      1525..1604
                     /label=BBa_B0010
     stem_loop       1620..1639
                     /label=T7 TE
     misc_feature    1646..1646
                     /label=stop
     polyA_site      1640..1653
                     /label=polya
     misc_feature    1613..1653
                     /label=BBa_B0012
     terminator      1613..1653
                     /label=BBa_B0012
ORIGIN
        1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac
       61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagaaaga ggagaaatac
      121 tagatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
      181 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
      241 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
      301 accctcgtga ccaccttcgg ctacggcctg caatgcttcg cccgctaccc cgaccacatg
      361 aagctgcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
      421 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
      481 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
      541 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
      601 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
      661 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
      721 cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
      781 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
      841 taataatact agagtcacac aggaaagtac tagatgacta taatgataaa aaaatcggat
      901 tttttggcaa ttccatcgga ggagtataaa ggtattctaa gtcttcgtta tcaagtgttt
      961 aagcaaagac ttgagtggga cttagttgta gaaaataacc ttgaatcaga tgagtatgat
     1021 aactcaaatg cagaatatat ttatgcttgt gatgatactg aaaatgtaag tggatgctgg
     1081 cgtttattac ctacaacagg tgattatatg ctgaaaagtg tttttcctga attgcttggt
     1141 caacagagtg ctcccaaaga tcctaatata gtcgaattaa gtcgttttgc tgtaggtaaa
     1201 aatagctcaa agataaataa ctctgctagt gaaattacaa tgaaactatt tgaagctata
     1261 tataaacacg ctgttagtca aggtattaca gaatatgtaa cagtaacatc aacagcaata
     1321 gagcgatttt taaagcgtat taaagttcct tgtcatcgta ttggagacaa agaaattcat
     1381 gtattaggtg atactaaatc ggttgtattg tctatgccta ttaatgaaca gtttaaaaaa
     1441 gcagtcttaa atgctgcaaa cgacgaaaac tacgctttag tagcttaata actctgatag
     1501 tgctagtgta gatctctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1561 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1621 gctcaccttc gggtgggcct ttctgcgttt ata
//