LOCUS       BBa_J06574               896 bp    DNA     linear       09-May-2024
DEFINITION  Construction intermediate: cI857 (Lamda II) with RBS and forward 
            terminator (B0034.J06503.B0014)
ACCESSION   BBa_J06574
VERSION     BBa_J06574.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..729
                     /label=cI857
     misc_feature    730..762
                     /label=LVA
     misc_feature    217..217
                     /label=G->A (857)
     misc_feature    370..370
                     /label=G->A (ind-)
     misc_feature    769..793
                     /label=barcode from LacI-LVA (BBa_C0012)
     CDS             19..793
                     /label=BBa_J06503
     stem_loop       809..828
                     /label=T7 TE
     misc_feature    835..835
                     /label=stop
     polyA_site      829..842
                     /label=polya
     misc_feature    802..842
                     /label=BBa_B0012
     terminator      802..842
                     /label=BBa_B0012
     stem_loop       863..885
                     /label=stem_loop
     misc_feature    851..896
                     /label=BBa_B0011
     terminator      851..896
                     /label=BBa_B0011
ORIGIN
        1 aaagaggaga aatactagat gagcacaaaa aagaaaccat taacacaaga gcagcttgag
       61 gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag
      121 gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc
      181 atcaatgcat taaatgctta taacgccgca ttgcttacaa aaattctcaa agttagcgtt
      241 gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg
      301 cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg
      361 ttctcaccta agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc
      421 aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca
      481 acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct
      541 gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa
      601 ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc
      661 ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag
      721 acgtttggcg ctgcaaacga cgaaaactac gctttagtag cttaataacg ctgatagtgc
      781 tagtgtagat cgctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
      841 tatactagag agagaatata aaaagccaga ttattaatcc ggctttttta ttattt
//