LOCUS       BBa_J06621              1789 bp    DNA     linear       21-May-2024
DEFINITION  Construction intermediate: 434 cI QPI with EYFP (Q04520.E0430)
ACCESSION   BBa_J06621
VERSION     BBa_J06621.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..687
                     /label=cI 434
     misc_feature    649..687
                     /label=LVA
     misc_feature    19..687
                     /label=BBa_C0052
     misc_feature    688..712
                     /label=Help:Barcodes
     CDS             19..687
                     /label=BBa_C0052
     stem_loop       732..775
                     /label=stem_loop
     misc_feature    721..800
                     /label=BBa_B0010
     terminator      721..800
                     /label=BBa_B0010
     stem_loop       816..835
                     /label=T7 TE
     misc_feature    842..842
                     /label=stop
     polyA_site      836..849
                     /label=polya
     misc_feature    809..849
                     /label=BBa_B0012
     terminator      809..849
                     /label=BBa_B0012
     misc_feature    858..900
                     /label=OR2
     misc_feature    865..878
                     /label=OR2
     misc_feature    887..900
                     /label=OR1
     promoter        858..863
                     /label=-35
     promoter        881..886
                     /label=-10
     promoter        859..864
                     /label=-35
     misc_feature    892..894
                     /label=start
     misc_feature    858..903
                     /label=BBa_R0052
     promoter        858..903
                     /label=BBa_R0052
     misc_feature    916..919
                     /label=conserved
     RBS             912..923
                     /label=BBa_B0034
     CDS             930..1652
                     /label=BBa_E0030
     stem_loop       1672..1715
                     /label=stem_loop
     misc_feature    1661..1740
                     /label=BBa_B0010
     terminator      1661..1740
                     /label=BBa_B0010
     stem_loop       1756..1775
                     /label=T7 TE
     misc_feature    1782..1782
                     /label=stop
     polyA_site      1776..1789
                     /label=polya
     misc_feature    1749..1789
                     /label=BBa_B0012
     terminator      1749..1789
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gagtatttct tccagggtaa aaagcaaaag aatccagctt
       61 ggacttaacc aggctgaact tgctcaaaag gtggggacta cccagcagtc tatagagcag
      121 ctcgaaaacg gtaaaactaa gcgaccacgc tttttaccag aacttgcgtc agctcttggc
      181 gtaagtgttg actggctgct caatggcacc tctgattcga atgttagatt tgttgggcac
      241 gttgagccca aagggaaata tccattgatt agcatggtta gagctggttc gtggtgtgaa
      301 gcttgtgaac cctacgatat caaggacatt gatgaatggt atgacagtga cgttaactta
      361 ttaggcaatg gattctggct gaaggttgaa ggtgattcca tgacctcacc tgtaggtcaa
      421 agcatccctg aaggtcatat ggtgttagta gatactggac gggagccagt gaatggaagc
      481 cttgttgtag ccaaactgac tgacgcgaac gaagcaacat tcaagaaact ggtcatagat
      541 ggcggtcaga agtacctgaa aggcctgaat ccttcatggc ctatgactcc tatcaacgga
      601 aactgcaaga ttatcggtgt tgtcgtggaa gcgagggtaa aattcgtagc tgcaaacgac
      661 gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag
      721 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
      781 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      841 gcgtttatat actagagttg acaaacaaga tacattgtat gaaaatacaa gaaagtttgt
      901 tgatactaga gaaagaggag aaatactaga tggtgagcaa gggcgaggag ctgttcaccg
      961 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     1021 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     1081 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcaat
     1141 gcttcgcccg ctaccccgac cacatgaagc tgcacgactt cttcaagtcc gccatgcccg
     1201 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     1261 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     1321 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     1381 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     1441 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     1501 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag
     1561 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     1621 ctctcggcat ggacgagctg tacaagtaat aatactagag ccaggcatca aataaaacga
     1681 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1741 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//