LOCUS       BBa_J06623              1844 bp    DNA     linear       06-May-2024
DEFINITION  Construction intermediate: 434 cI QPI with Venus YFP 
            (Q04520.J06700)
ACCESSION   BBa_J06623
VERSION     BBa_J06623.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..687
                     /label=cI 434
     misc_feature    649..687
                     /label=LVA
     misc_feature    19..687
                     /label=BBa_C0052
     misc_feature    688..712
                     /label=Help:Barcodes
     CDS             19..687
                     /label=BBa_C0052
     stem_loop       732..775
                     /label=stem_loop
     misc_feature    721..800
                     /label=BBa_B0010
     terminator      721..800
                     /label=BBa_B0010
     stem_loop       816..835
                     /label=T7 TE
     misc_feature    842..842
                     /label=stop
     polyA_site      836..849
                     /label=polya
     misc_feature    809..849
                     /label=BBa_B0012
     terminator      809..849
                     /label=BBa_B0012
     misc_feature    858..900
                     /label=OR2
     misc_feature    865..878
                     /label=OR2
     misc_feature    887..900
                     /label=OR1
     promoter        858..863
                     /label=-35
     promoter        881..886
                     /label=-10
     promoter        859..864
                     /label=-35
     misc_feature    892..894
                     /label=start
     misc_feature    858..903
                     /label=BBa_R0052
     promoter        858..903
                     /label=BBa_R0052
     misc_feature    916..919
                     /label=conserved
     RBS             912..923
                     /label=BBa_B0034
     CDS             930..1676
                     /label=Venus YFP
     misc_feature    930..944
                     /label=MATSG linker
     misc_feature    1662..1676
                     /label=GSGTA linker
     misc_feature    1683..1707
                     /label=Help:Barcodes
     misc_feature    930..1682
                     /label=BBa_E2030
     stem_loop       1727..1770
                     /label=stem_loop
     misc_feature    1716..1795
                     /label=BBa_B0010
     terminator      1716..1795
                     /label=BBa_B0010
     stem_loop       1811..1830
                     /label=T7 TE
     misc_feature    1837..1837
                     /label=stop
     polyA_site      1831..1844
                     /label=polya
     misc_feature    1804..1844
                     /label=BBa_B0012
     terminator      1804..1844
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gagtatttct tccagggtaa aaagcaaaag aatccagctt
       61 ggacttaacc aggctgaact tgctcaaaag gtggggacta cccagcagtc tatagagcag
      121 ctcgaaaacg gtaaaactaa gcgaccacgc tttttaccag aacttgcgtc agctcttggc
      181 gtaagtgttg actggctgct caatggcacc tctgattcga atgttagatt tgttgggcac
      241 gttgagccca aagggaaata tccattgatt agcatggtta gagctggttc gtggtgtgaa
      301 gcttgtgaac cctacgatat caaggacatt gatgaatggt atgacagtga cgttaactta
      361 ttaggcaatg gattctggct gaaggttgaa ggtgattcca tgacctcacc tgtaggtcaa
      421 agcatccctg aaggtcatat ggtgttagta gatactggac gggagccagt gaatggaagc
      481 cttgttgtag ccaaactgac tgacgcgaac gaagcaacat tcaagaaact ggtcatagat
      541 ggcggtcaga agtacctgaa aggcctgaat ccttcatggc ctatgactcc tatcaacgga
      601 aactgcaaga ttatcggtgt tgtcgtggaa gcgagggtaa aattcgtagc tgcaaacgac
      661 gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag
      721 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
      781 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      841 gcgtttatat actagagttg acaaacaaga tacattgtat gaaaatacaa gaaagtttgt
      901 tgatactaga gaaagaggag aaatactaga tggcaactag cggcatggtt agtaaaggag
      961 aagaactttt cactggagtt gtcccaattt tagttgaact agatggcgac gtgaacggtc
     1021 ataagttcag tgtctccggc gaaggtgagg gtgatgcaac gtacggtaag ttaactttga
     1081 agttaatatg tacaaccggc aagctgcctg ttccctggcc taccctggtg acaacgttag
     1141 gttatgggtt gatgtgcttt gctagatacc cagatcacat gaaaaggcat gacttcttta
     1201 aatctgcaat gccagaaggt tacgtccaag aacgtactat tttctttaaa gatgacggta
     1261 attataaaac tagggctgaa gttaaattcg aaggtgacac acttgtaaat cgaatagagt
     1321 taaaggggat tgatttcaaa gaggatggta atattctagg ccataaactt gaatataact
     1381 ataattcaca caacgtttac attaccgccg acaagcagaa gaatggaatc aaagccaatt
     1441 ttaagattag acacaatatt gaggatggtg gagtacagct tgctgatcat taccaacaaa
     1501 ataccccgat cggtgatgga ccagttttgc tacccgataa ccattatctg tcctatcaaa
     1561 gcaaattgtc aaaagatcct aacgaaaaaa gagaccacat ggtactcttg gaatttgtaa
     1621 cagctgctgg gattacacat ggcatggatg aactatacaa aggttctggt accgcataat
     1681 aaccctgata gtgctagtgt agatccctac tagagccagg catcaaataa aacgaaaggc
     1741 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     1801 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//