LOCUS       BBa_J06627              1780 bp    DNA     linear       10-May-2024
DEFINITION  Construction intermediate: 434 cI QPI with mCherry, bact 
            (Q04520.J06702)
ACCESSION   BBa_J06627
VERSION     BBa_J06627.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..687
                     /label=cI 434
     misc_feature    649..687
                     /label=LVA
     misc_feature    19..687
                     /label=BBa_C0052
     misc_feature    688..712
                     /label=Help:Barcodes
     CDS             19..687
                     /label=BBa_C0052
     stem_loop       732..775
                     /label=stem_loop
     misc_feature    721..800
                     /label=BBa_B0010
     terminator      721..800
                     /label=BBa_B0010
     stem_loop       816..835
                     /label=T7 TE
     misc_feature    842..842
                     /label=stop
     polyA_site      836..849
                     /label=polya
     misc_feature    809..849
                     /label=BBa_B0012
     terminator      809..849
                     /label=BBa_B0012
     misc_feature    858..900
                     /label=OR2
     misc_feature    865..878
                     /label=OR2
     misc_feature    887..900
                     /label=OR1
     promoter        858..863
                     /label=-35
     promoter        881..886
                     /label=-10
     promoter        859..864
                     /label=-35
     misc_feature    892..894
                     /label=start
     misc_feature    858..903
                     /label=BBa_R0052
     promoter        858..903
                     /label=BBa_R0052
     misc_feature    916..919
                     /label=conserved
     RBS             912..923
                     /label=BBa_B0034
     CDS             930..1640
                     /label=mCherry
     misc_feature    1281..1281
                     /label=C->T (removing PstI site)
     misc_feature    930..1643
                     /label=BBa_J06504
     stem_loop       1663..1706
                     /label=stem_loop
     misc_feature    1652..1731
                     /label=BBa_B0010
     terminator      1652..1731
                     /label=BBa_B0010
     stem_loop       1747..1766
                     /label=T7 TE
     misc_feature    1773..1773
                     /label=stop
     polyA_site      1767..1780
                     /label=polya
     misc_feature    1740..1780
                     /label=BBa_B0012
     terminator      1740..1780
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gagtatttct tccagggtaa aaagcaaaag aatccagctt
       61 ggacttaacc aggctgaact tgctcaaaag gtggggacta cccagcagtc tatagagcag
      121 ctcgaaaacg gtaaaactaa gcgaccacgc tttttaccag aacttgcgtc agctcttggc
      181 gtaagtgttg actggctgct caatggcacc tctgattcga atgttagatt tgttgggcac
      241 gttgagccca aagggaaata tccattgatt agcatggtta gagctggttc gtggtgtgaa
      301 gcttgtgaac cctacgatat caaggacatt gatgaatggt atgacagtga cgttaactta
      361 ttaggcaatg gattctggct gaaggttgaa ggtgattcca tgacctcacc tgtaggtcaa
      421 agcatccctg aaggtcatat ggtgttagta gatactggac gggagccagt gaatggaagc
      481 cttgttgtag ccaaactgac tgacgcgaac gaagcaacat tcaagaaact ggtcatagat
      541 ggcggtcaga agtacctgaa aggcctgaat ccttcatggc ctatgactcc tatcaacgga
      601 aactgcaaga ttatcggtgt tgtcgtggaa gcgagggtaa aattcgtagc tgcaaacgac
      661 gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag
      721 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
      781 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      841 gcgtttatat actagagttg acaaacaaga tacattgtat gaaaatacaa gaaagtttgt
      901 tgatactaga gaaagaggag aaatactaga tggtgagcaa gggcgaggag gataacatgg
      961 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     1021 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     1081 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     1141 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     1201 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     1261 ccgtgaccca ggactcctcc ttgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     1321 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     1381 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     1441 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     1501 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     1561 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     1621 tggacgagct gtacaagtaa taatactaga gccaggcatc aaataaaacg aaaggctcag
     1681 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     1741 cacactggct caccttcggg tgggcctttc tgcgtttata
//