LOCUS BBa_J06951 3355 bp DNA linear 10-May-2024 DEFINITION Lambda cI/LacI ts (mut 241) RS flip-flop ACCESSION BBa_J06951 VERSION BBa_J06951.1 FEATURES Location/Qualifiers promoter 38..43 /label=-10 promoter 15..20 /label=-35 misc_feature 25..41 /label=OR1 misc_feature 1..17 /label=OR2 misc_feature 1..49 /label=BBa_R0051 promoter 1..49 /label=BBa_R0051 misc_feature 62..65 /label=conserved RBS 58..69 /label=BBa_B0034 CDS 76..1203 /label=LacI(ts)-LVA misc_feature 1165..1203 /label=LVA misc_feature 1204..1228 /label=barcode from LacI (BBa_C0012) misc_feature 805..805 /label=TS mutation (G->A) CDS 76..1228 /label=BBa_J06500 stem_loop 1248..1291 /label=stem_loop misc_feature 1237..1316 /label=BBa_B0010 terminator 1237..1316 /label=BBa_B0010 stem_loop 1332..1351 /label=T7 TE misc_feature 1358..1358 /label=stop polyA_site 1352..1365 /label=polya misc_feature 1325..1365 /label=BBa_B0012 terminator 1325..1365 /label=BBa_B0012 misc_feature 1376..1392 /label=lac O1 promoter 1393..1398 /label=-35 misc_feature 1399..1415 /label=lac O1 promoter 1416..1421 /label=-10 misc_feature 1374..1427 /label=BBa_R0011 promoter 1374..1427 /label=BBa_R0011 misc_feature 1441..1444 /label=conserved RBS 1437..1448 /label=BBa_B0034 CDS 1458..2165 /label=cI lambda misc_feature 2166..2198 /label=LVA misc_feature 2205..2229 /label=Help:Barcodes CDS 1455..2204 /label=BBa_C0051 stem_loop 2249..2292 /label=stem_loop misc_feature 2238..2317 /label=BBa_B0010 terminator 2238..2317 /label=BBa_B0010 stem_loop 2333..2352 /label=T7 TE misc_feature 2359..2359 /label=stop polyA_site 2353..2366 /label=polya misc_feature 2326..2366 /label=BBa_B0012 terminator 2326..2366 /label=BBa_B0012 promoter 2412..2417 /label=-10 promoter 2389..2394 /label=-35 misc_feature 2399..2415 /label=OR1 misc_feature 2375..2391 /label=OR2 misc_feature 2375..2423 /label=BBa_R0051 promoter 2375..2423 /label=BBa_R0051 misc_feature 2436..2439 /label=conserved RBS 2432..2443 /label=BBa_B0034 misc_feature 2450..2464 /label=MATSG linker misc_feature 3173..3187 /label=GSGTA linker CDS 2450..3187 /label=mCherry misc_feature 3194..3218 /label=Help:Barcodes misc_feature 2450..3193 /label=BBa_E2060 stem_loop 3238..3281 /label=stem_loop misc_feature 3227..3306 /label=BBa_B0010 terminator 3227..3306 /label=BBa_B0010 stem_loop 3322..3341 /label=T7 TE misc_feature 3348..3348 /label=stop polyA_site 3342..3355 /label=polya misc_feature 3315..3355 /label=BBa_B0012 terminator 3315..3355 /label=BBa_B0012 ORIGIN 1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa 61 gaggagaaat actagatggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat 121 gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg 181 aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg 241 gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc 301 ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc 361 agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac 421 aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat 481 gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac 541 cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag 601 catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc 661 tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg 721 atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg 781 ctgaatgagg gcatcgttcc cactacgatg ctggttgcca acgatcagat ggcgctgggc 841 gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga 901 tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat 961 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg 1021 gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc 1081 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag 1141 gtttcccgac tggaaagcgg gcaggctgca aacgacgaaa actacgcttt agtagcttaa 1201 taactctgat agtgctagtg tagatctcta ctagagccag gcatcaaata aaacgaaagg 1261 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 1321 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagaattgtg 1381 agcggataac aattgacatt gtgagcggat aacaagatac tgagcacata ctagagaaag 1441 aggagaaata ctagatgagc acaaaaaaga aaccattaac acaagagcag cttgaggacg 1501 cacgtcgcct taaagcaatt tatgaaaaaa agaaaaatga acttggctta tcccaggaat 1561 ctgtcgcaga caagatgggg atggggcagt caggcgttgg tgctttattt aatggcatca 1621 atgcattaaa tgcttataac gccgcattgc ttgcaaaaat tctcaaagtt agcgttgaag 1681 aatttagccc ttcaatcgcc agagaaatct acgagatgta tgaagcggtt agtatgcagc 1741 cgtcacttag aagtgagtat gagtaccctg ttttttctca tgttcaggca gggatgttct 1801 cacctgagct tagaaccttt accaaaggtg atgcggagag atgggtaagc acaaccaaaa 1861 aagccagtga ttctgcattc tggcttgagg ttgaaggtaa ttccatgacc gcaccaacag 1921 gctccaagcc aagctttcct gacggaatgt taattctcgt tgaccctgag caggctgttg 1981 agccaggtga tttctgcata gccagacttg ggggtgatga gtttaccttc aagaaactga 2041 tcagggatag cggtcaggtg tttttacaac cactaaaccc acagtaccca atgatcccat 2101 gcaatgagag ttgttccgtt gtggggaaag ttatcgctag tcagtggcct gaagagacgt 2161 ttggcgctgc aaacgacgaa aactacgctt tagtagctta ataacgctga tagtgctagt 2221 gtagatcgct actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 2281 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 2341 ttcgggtggg cctttctgcg tttatatact agagtaacac cgtgcgtgtt gactatttta 2401 cctctggcgg tgataatggt tgctactaga gaaagaggag aaatactaga tggcaactag 2461 cggcatggtt agtaaaggag aagaaaataa catggcaatc attaaggagt tcatgagatt 2521 caaagttcac atggaaggtt ctgtaaatgg acatgaattt gaaatagaag gtgaaggaga 2581 aggaaggcct tatgaaggaa cccaaaccgc gaagctaaaa gttactaagg gtggcccatt 2641 accatttgca tgggatatcc ttagccctca attcatgtat gggtcaaagg cttatgtcaa 2701 gcaccccgcc gacattccag actatctaaa gttatctttt cccgaagggt ttaagtggga 2761 gcgtgtgatg aacttcgaag acggtggcgt ggtaacagtg actcaggatt cgtccctgca 2821 agatggtgaa tttatctaca aagtcaaatt aagaggaact aactttccat ctgacggccc 2881 ggttatgcaa aaaaagacaa tgggctggga ggcctcctca gaacgaatgt accctgaaga 2941 tggtgccttg aagggtgaga ttaaacaaag attgaaattg aaagatggtg gacattatga 3001 cgctgaggtt aaaacgacat acaaagctaa gaaacctgtc cagctcccag gtgcttacaa 3061 tgtaaatata aaacttgata ttacatcaca taatgaagat tatacgatag ttgaacaata 3121 cgaaagggct gaggggagac atagtactgg tggcatggat gaactataca aaggttctgg 3181 taccgcataa taacactgat agtgctagtg tagatcacta ctagagccag gcatcaaata 3241 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 3301 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata //