LOCUS BBa_J07001 3229 bp DNA linear 02-May-2024 DEFINITION abandoned test construct ACCESSION BBa_J07001 VERSION BBa_J07001.1 FEATURES Location/Qualifiers misc_feature 1..3 /label=OR3 misc_feature 11..28 /label=OR2 misc_feature 34..51 /label=OR1 promoter 18..23 /label=-35 promoter 8..13 /label=-35 promoter 30..35 /label=-10 misc_feature 1..54 /label=BBa_R0053 promoter 1..54 /label=BBa_R0053 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 1170..1208 /label=LVA CDS 81..1208 /label=lacI-LVA misc_feature 81..1208 /label=BBa_C0012 misc_feature 1209..1233 /label=Help:Barcodes CDS 81..1208 /label=BBa_C0012 stem_loop 1253..1296 /label=stem_loop misc_feature 1242..1321 /label=BBa_B0010 terminator 1242..1321 /label=BBa_B0010 stem_loop 1337..1356 /label=T7 TE misc_feature 1363..1363 /label=stop polyA_site 1357..1370 /label=polya misc_feature 1330..1370 /label=BBa_B0012 terminator 1330..1370 /label=BBa_B0012 misc_feature 1381..1397 /label=lac O1 promoter 1398..1403 /label=-35 misc_feature 1404..1420 /label=lac O1 promoter 1421..1426 /label=-10 misc_feature 1379..1432 /label=BBa_R0011 promoter 1379..1432 /label=BBa_R0011 misc_feature 1446..1449 /label=conserved RBS 1442..1453 /label=BBa_B0034 CDS 1463..2079 /label=tetR misc_feature 2080..2113 /label=SsrA misc_feature 2120..2144 /label=Help:Barcodes CDS 1460..2119 /label=BBa_C0040 stem_loop 2164..2207 /label=stem_loop misc_feature 2153..2232 /label=BBa_B0010 terminator 2153..2232 /label=BBa_B0010 stem_loop 2248..2267 /label=T7 TE misc_feature 2274..2274 /label=stop polyA_site 2268..2281 /label=polya misc_feature 2241..2281 /label=BBa_B0012 terminator 2241..2281 /label=BBa_B0012 misc_feature 2290..2308 /label=TetR 1 misc_feature 2290..2343 /label=BBa_R0040 promoter 2309..2314 /label=-35 misc_feature 2315..2333 /label=TetR 2 promoter 2332..2337 /label=-10 promoter 2290..2343 /label=BBa_R0040 misc_feature 2356..2359 /label=conserved RBS 2352..2363 /label=BBa_B0034 CDS 2370..3092 /label=BBa_E0030 stem_loop 3112..3155 /label=stem_loop misc_feature 3101..3180 /label=BBa_B0010 terminator 3101..3180 /label=BBa_B0010 stem_loop 3196..3215 /label=T7 TE misc_feature 3222..3222 /label=stop polyA_site 3216..3229 /label=polya misc_feature 3189..3229 /label=BBa_B0012 terminator 3189..3229 /label=BBa_B0012 ORIGIN 1 aataaacttg actaaagatt cctttagtag ataatttaag tgttctttaa tttctactag 61 agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag 121 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt 181 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc 241 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc 301 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg 361 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg 421 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc 481 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct 541 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg 601 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt 661 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc 721 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc 781 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc 841 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag 901 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac 961 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc 1021 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg 1081 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 1141 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag 1201 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg 1261 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 1321 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagaa 1381 ttgtgagcgg ataacaattg acattgtgag cggataacaa gatactgagc acatactaga 1441 gaaagaggag aaatactaga tgtccagatt agataaaagt aaagtgatta acagcgcatt 1501 agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct 1561 aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc 1621 cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag 1681 ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg 1741 cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga 1801 aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact 1861 cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc 1921 taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac gacaagctat 1981 cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat 2041 atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc gctgcaaacg acgaaaacta 2101 cgctttagta gcttaataac actgatagtg ctagtgtaga tcactactag agccaggcat 2161 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 2221 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 2281 atactagagt ccctatcagt gatagagatt gacatcccta tcagtgatag agatactgag 2341 cactactaga gaaagaggag aaatactaga tggtgagcaa gggcgaggag ctgttcaccg 2401 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt 2461 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca 2521 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcaat 2581 gcttcgcccg ctaccccgac cacatgaagc tgcacgactt cttcaagtcc gccatgcccg 2641 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg 2701 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact 2761 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg 2821 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca 2881 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg 2941 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag 3001 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca 3061 ctctcggcat ggacgagctg tacaagtaat aatactagag ccaggcatca aataaaacga 3121 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 3181 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata //