LOCUS       BBa_J07038               508 bp    DNA     linear       19-May-2024
DEFINITION  Threshold: Activation module (cI anti-sense)
ACCESSION   BBa_J07038
VERSION     BBa_J07038.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    264..371
                     /label=antisense region for I1030
     misc_feature    209..263
                     /label=LacO-1
     misc_feature    209..371
                     /label=BBa_I1031
     misc_feature    209..371
                     /label=BBa_I1031
     stem_loop       391..434
                     /label=stem_loop
     misc_feature    380..459
                     /label=BBa_B0010
     terminator      380..459
                     /label=BBa_B0010
     stem_loop       475..494
                     /label=T7 TE
     misc_feature    501..501
                     /label=stop
     polyA_site      495..508
                     /label=polya
     misc_feature    468..508
                     /label=BBa_B0012
     terminator      468..508
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat aaatgtgagc ggataacatt gacattgtga
      241 gcggataaca agatactgag cacttcataa attgctttaa ggcgacgtgc gtcctcaagc
      301 tgctcttgtg ttaatggttt cttttttgtc gacatcgaac cggtttcctg tcgttgctgt
      361 tgtgcaaagc ttactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      421 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
      481 ccttcgggtg ggcctttctg cgtttata
//