LOCUS       BBa_J07040               515 bp    DNA     linear       19-May-2024
DEFINITION  Threshold: Activation module (IS10-type cI antisense)
ACCESSION   BBa_J07040
VERSION     BBa_J07040.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    264..298
                     /label=Reverse Complement to cI mRNA
     stem_loop       287..307
                     /label=stem_loop
     misc_feature    276..281
                     /label=Reverse Complement RBS
     misc_feature    264..271
                     /label=reverse complement to cI cds
     misc_feature    269..271
                     /label=start
     stem_loop       266..328
                     /label=stem_loop
     misc_feature    266..268
                     /label=added codon (Cys)
     misc_feature    264..264
                     /label=start
     misc_feature    209..263
                     /label=LacO-1
     misc_feature    209..378
                     /label=BBa_I1033
     misc_feature    209..378
                     /label=BBa_I1033
     stem_loop       398..441
                     /label=stem_loop
     misc_feature    387..466
                     /label=BBa_B0010
     terminator      387..466
                     /label=BBa_B0010
     stem_loop       482..501
                     /label=T7 TE
     misc_feature    508..508
                     /label=stop
     polyA_site      502..515
                     /label=polya
     misc_feature    475..515
                     /label=BBa_B0012
     terminator      475..515
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat aaatgtgagc ggataacatt gacattgtga
      241 gcggataaca agatactgag cacgagcaca tcttgttgtc tgattattga tttttcgcga
      301 aaccatttga tcatatgaca agatgtgtat ccaccttaac ttaatgattt ttaccaaaat
      361 cattagggga ttcatcagta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
      421 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
      481 tggctcacct tcgggtgggc ctttctgcgt ttata
//