LOCUS       BBa_J07066               955 bp    DNA     linear       13-May-2024
DEFINITION  FecA Promoter-RFP test construct
ACCESSION   BBa_J07066
VERSION     BBa_J07066.1  
FEATURES             Location/Qualifiers
     misc_feature    1..60
                     /label=-59 to -1 region
     misc_feature    25..39
                     /label=Fur Box (binds Fur)
     misc_feature    70..76
                     /label=FecI binding region
     promoter        1..86
                     /label=BBa_J07019
     misc_feature    99..102
                     /label=conserved
     RBS             95..106
                     /label=BBa_B0034
     CDS             113..787
                     /label=mrfp1
     misc_feature    794..818
                     /label=Help:Barcodes
     CDS             113..793
                     /label=BBa_E1010
     stem_loop       838..881
                     /label=stem_loop
     misc_feature    827..906
                     /label=BBa_B0010
     terminator      827..906
                     /label=BBa_B0010
     stem_loop       922..941
                     /label=T7 TE
     misc_feature    948..948
                     /label=stop
     polyA_site      942..955
                     /label=polya
     misc_feature    915..955
                     /label=BBa_B0012
     terminator      915..955
                     /label=BBa_B0012
ORIGIN
        1 tggataaaca tttcaccact gtaaggaaaa taattcttat ttcgattgtc ctttttaccc
       61 ttctcgttcg actcatagct gaacactact agagaaagag gagaaatact agatggcttc
      121 ctccgaagac gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa
      181 cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac
      241 cgctaaactg aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc
      301 gcagttccag tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct
      361 gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg
      421 tgttgttacc gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa
      481 actgcgtggt accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg
      541 ggaagcttcc accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat
      601 gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc
      661 taaaaaaccg gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc
      721 ccacaacgaa gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac
      781 cggtgcttaa taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata
      841 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
      901 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//