LOCUS       BBa_J09771               885 bp    DNA     linear       14-May-2024
DEFINITION  Constitutive tetR device, upstream terminator
ACCESSION   BBa_J09771
VERSION     BBa_J09771.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     terminator      1..129
                     /label=BBa_B0015
     misc_feature    138..174
                     /label=P(Lac) IQ
     promoter        140..145
                     /label=-35
     promoter        163..168
                     /label=-10
     promoter        138..174
                     /label=BBa_I14032
     misc_feature    187..190
                     /label=conserved
     RBS             183..194
                     /label=BBa_B0034
     CDS             204..820
                     /label=tetR
     misc_feature    821..854
                     /label=SsrA
     misc_feature    861..885
                     /label=Help:Barcodes
     CDS             201..885
                     /label=BBa_C0040
     misc_feature    138..885
                     /label=BBa_J09761
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagtgg tgcaaaacct ttcgcggtat ggcatgatag cgcctactag
      181 agaaagagga gaaatactag atgtccagat tagataaaag taaagtgatt aacagcgcat
      241 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc
      301 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg
      361 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa
      421 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc
      481 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg
      541 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac
      601 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg
      661 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta
      721 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca
      781 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact
      841 acgctttagt agcttaataa cactgatagt gctagtgtag atcac
//