LOCUS       BBa_J100001             1357 bp    DNA     linear       13-Jun-2024
DEFINITION  pTet+RBS+Cre2SAT1Clause+pLpp+tRNA CCACU
ACCESSION   BBa_J100001
VERSION     BBa_J100001.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..83
                     /label=Start
     misc_feature    84..91
                     /label=AsiSI site
     misc_feature    93..103
                     /label=1 Clause 2-SAT
     misc_feature    108..115
                     /label=AscI site
     CDS             116..1149
                     /label=Remainder of Cre Recombinase
     CDS             81..1149
                     /label=BBa_J100000
     promoter        1158..1206
                     /label=BBa_K199106
     misc_feature    1215..1225
                     /label=5' Context
     misc_feature    1258..1266
                     /label=Anticodon Loop
     misc_feature    1318..1357
                     /label=3' Context
     misc_feature    1215..1357
                     /label=BBa_K199002
     misc_feature    1158..1357
                     /label=BBa_S04333
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggcgatcg caccactgct agttctgggc gcgcctccaa
      121 tttactgacc gtacaccaaa atttgcctgc attaccggtc gatgcaacga gtgatgaggt
      181 tcgcaagaac ctgatggaca tgttcaggga tcgccaggcg ttttctgagc atacctggaa
      241 aatgcttctg tccgtttgcc ggtcgtgggc ggcatggtgc aagttgaata accggaaatg
      301 gtttcccgca gaacctgaag atgttcgcga ttatcttcta tatcttcagg cgcgcggtct
      361 ggcagtaaaa actatccagc aacatttggg ccagctaaac atgcttcatc gtcggtccgg
      421 gctgccacga ccaagtgaca gcaatgctgt ttcactggtt atgcggcgga tccgaaaaga
      481 aaacgttgat gccggtgaac gtgcaaaaca ggctctagcg ttcgaacgca ctgatttcga
      541 ccaggttcgt tcactcatgg aaaatagcga tcgctgccag gatatacgta atctggcatt
      601 tctggggatt gcttataaca ccctgttacg tatagccgaa attgccagga tcagggttaa
      661 agatatctca cgtactgacg gtgggagaat gttaatccat attggcagaa cgaaaacgct
      721 ggttagcacc gcaggtgtag agaaggcact tagcctgggg gtaactaaac tggtcgagcg
      781 atggatttcc gtctctggtg tagctgatga tccgaataac tacctgtttt gccgggtcag
      841 aaaaaatggt gttgccgcgc catctgccac cagccagcta tcaactcgcg ccctggaagg
      901 gatttttgaa gcaactcatc gattgattta cggcgctaag gatgactctg gtcagagata
      961 cctggcctgg tctggacaca gtgcccgtgt cggagccgcg cgagatatgg cccgcgctgg
     1021 agtttcaata ccggagatca tgcaagctgg tggctggacc aatgtaaata ttgtcatgaa
     1081 ctatatccgt aacctggata gtgaaacagg ggcaatggtg cgcctgctgg aagatggcga
     1141 ttaagaattt actagagatc aaaaaaatat tctcaacata aaaaactttg tgtaatactt
     1201 gtaacgtact agagggatcc aattcggaga gatgccggag cggctgaacg gaccggtcta
     1261 gtggacaccg gagtaggggc aactctaccg ggggttcaaa tccccctctc tccgccactg
     1321 catatcctta gcgaaagcta aggatttttt ttaagct
//