LOCUS       BBa_J100083             1081 bp    DNA     linear       18-May-2024
DEFINITION  LuxI Long + RBS + GFP
ACCESSION   BBa_J100083
VERSION     BBa_J100083.1  
FEATURES             Location/Qualifiers
     misc_feature    94..109
                     /label=ArcA binding site 2
     misc_feature    127..144
                     /label=arcA binding site 1
     misc_feature    84..109
                     /label=CRP binding site
     misc_feature    145..165
                     /label=LuxR binding site
     misc_feature    188..188
                     /label=-10
     misc_feature    197..197
                     /label=+1
     promoter        1..197
                     /label=BBa_J100074
     RBS             206..218
                     /label=RBS-3\Weak
     RBS             212..215
                     /label=RBS
     misc_feature    206..218
                     /label=BBa_B0032
     RBS             206..218
                     /label=BBa_B0032
     CDS             225..944
                     /label=GFP protein
     CDS             225..944
                     /label=BBa_E0040
     stem_loop       964..1007
                     /label=stem_loop
     misc_feature    953..1032
                     /label=BBa_B0010
     terminator      953..1032
                     /label=BBa_B0010
     stem_loop       1048..1067
                     /label=T7 TE
     misc_feature    1074..1074
                     /label=stop
     polyA_site      1068..1081
                     /label=polya
     misc_feature    1041..1081
                     /label=BBa_B0012
     terminator      1041..1081
                     /label=BBa_B0012
     misc_feature    206..1081
                     /label=BBa_E0240
ORIGIN
        1 catctcttta tccttaccta ttgtttgtcg caagttttgc gtgttatata tcattaaaac
       61 ggtaatggat tgacatttga ttctaataaa ttggattttt gtcacactat tgtatcgctg
      121 ggaatacaat tacttaacat aagcacctgt aggatcgtac aggtttacgc aagaaaatgg
      181 tttgttatag tcgaatatac tagagtcaca caggaaagta ctagatgcgt aaaggagaag
      241 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca
      301 aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat
      361 ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt
      421 atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga
      481 gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact
      541 acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa
      601 aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata
      661 actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca
      721 aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata
      781 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg
      841 ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag
      901 ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag agccaggcat
      961 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1021 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     1081 a
//