LOCUS       BBa_J100084             1042 bp    DNA     linear       22-May-2024
DEFINITION  CydAP Long + RBS + GFP
ACCESSION   BBa_J100084
VERSION     BBa_J100084.1  
FEATURES             Location/Qualifiers
     misc_feature    35..84
                     /label=arcA binding site
     misc_feature    91..105
                     /label=Fnr binding site 2
     misc_feature    143..158
                     /label=Fnr binding site 1
     misc_feature    116..116
                     /label=-35
     misc_feature    141..141
                     /label=-10
     misc_feature    151..151
                     /label=+1
     promoter        1..158
                     /label=BBa_J100075
     RBS             167..179
                     /label=RBS-3\Weak
     RBS             173..176
                     /label=RBS
     misc_feature    167..179
                     /label=BBa_B0032
     RBS             167..179
                     /label=BBa_B0032
     CDS             186..905
                     /label=GFP protein
     CDS             186..905
                     /label=BBa_E0040
     stem_loop       925..968
                     /label=stem_loop
     misc_feature    914..993
                     /label=BBa_B0010
     terminator      914..993
                     /label=BBa_B0010
     stem_loop       1009..1028
                     /label=T7 TE
     misc_feature    1035..1035
                     /label=stop
     polyA_site      1029..1042
                     /label=polya
     misc_feature    1002..1042
                     /label=BBa_B0012
     terminator      1002..1042
                     /label=BBa_B0012
     misc_feature    167..1042
                     /label=BBa_E0240
ORIGIN
        1 aactaatttc agccttataa ctcacacatt ttaaacataa atgtcagtaa agttacctta
       61 ttgaaacatg attaacataa tttgtaggaa ttgatattta tcaatgtata agtcttggaa
      121 atgggcatca aaaagagata aattgttctc gatcaaatta ctagagtcac acaggaaagt
      181 actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
      241 atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat
      301 acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa
      361 cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga
      421 aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat
      481 ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc
      541 ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac
      601 acaaattgga atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga
      661 atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc gttcaactag
      721 cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc
      781 attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg
      841 tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat
      901 aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
      961 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
     1021 ggtgggcctt tctgcgttta ta
//