LOCUS       BBa_J100086             1035 bp    DNA     linear       18-May-2024
DEFINITION  CydAP Short Promoter + RBS + GFP
ACCESSION   BBa_J100086
VERSION     BBa_J100086.1  
FEATURES             Location/Qualifiers
     misc_feature    151..151
                     /label=+1
     misc_feature    141..141
                     /label=-10
     misc_feature    35..84
                     /label=arcA binding site
     misc_feature    91..105
                     /label=Fnr binding site
     misc_feature    116..116
                     /label=-35
     promoter        1..151
                     /label=BBa_J100076
     RBS             160..172
                     /label=RBS-3\Weak
     RBS             166..169
                     /label=RBS
     misc_feature    160..172
                     /label=BBa_B0032
     RBS             160..172
                     /label=BBa_B0032
     CDS             179..898
                     /label=GFP protein
     CDS             179..898
                     /label=BBa_E0040
     stem_loop       918..961
                     /label=stem_loop
     misc_feature    907..986
                     /label=BBa_B0010
     terminator      907..986
                     /label=BBa_B0010
     stem_loop       1002..1021
                     /label=T7 TE
     misc_feature    1028..1028
                     /label=stop
     polyA_site      1022..1035
                     /label=polya
     misc_feature    995..1035
                     /label=BBa_B0012
     terminator      995..1035
                     /label=BBa_B0012
     misc_feature    160..1035
                     /label=BBa_E0240
ORIGIN
        1 aactaatttc agccttataa ctcacacatt ttaaacataa atgtcagtaa agttacctta
       61 ttgaaacatg attaacataa tttgtaggaa ttgatattta tcaatgtata agtcttggaa
      121 atgggcatca aaaagagata aattgttctc gtactagagt cacacaggaa agtactagat
      181 gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
      241 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
      301 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt
      361 cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca
      421 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa
      481 agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa
      541 tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt
      601 ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat
      661 caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca
      721 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct
      781 gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct
      841 tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca aataataata
      901 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
      961 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1021 ctttctgcgt ttata
//