LOCUS       BBa_J100173              245 bp    DNA     linear       01-Jun-2024
DEFINITION  Designed to optimize testing of fitness genes
ACCESSION   BBa_J100173
VERSION     BBa_J100173.1  
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /label=EcoRI
     misc_feature    7..169
                     /label=J100065 (theophylline riboswitch D)
     promoter        7..58
                     /label=T5 promoter
     misc_feature    59..78
                     /label=Lac operator
     misc_feature    79..108
                     /label=linkers and scars
     misc_feature    109..169
                     /label=aptamer and actuator of riboswitch
     misc_feature    167..169
                     /label=START
     misc_feature    171..176
                     /label=BsaI <--
     misc_feature    178..196
                     /label=stuffer
     misc_feature    197..202
                     /label=BsaI -->
     misc_feature    204..236
                     /label=LVA Degradation Tag
     misc_feature    237..239
                     /label=STOP
     misc_feature    240..245
                     /label=PstI
ORIGIN
        1 gaattcgaaa tcataaaaaa tttatttgct ttgtgagcgg ataacaatta taatagattc
       61 aattgtgagc ggataacaat tactagagat acgactcact ataggtaccg gtgataccag
      121 catcgtcttg atgcccttgg cagcaccctg ctaaggtaac aacaagatgt gagaccgcca
      181 gctttacggt ctttatggtc tctgctgcaa acgacgaaaa ctacgcttta gtagcttaac
      241 tgcag
//