BBa_J100201

BBa_J100201 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J100201
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Malcolm Campbell
Date created: 2014-10-23 11:00:00
Date modified: 2015-08-31 04:08:24

RBS (BD24) + GFP



Types
DnaRegion

Roles
engineered_region

Generator

Sequences BBa_J100201_sequence (Version 1)

Description

This is a composite of superfolder GFP and a BD24 RBS (part BBa J119028). The stop codon of the RBS and start codon of GFP overlap to increase effective translation. There is also an EcoRI recognition site preceding the RBS.

Notes

There are no BsaI sites.

Source

We assembled the part from preexisting parts which were isolated and cloned together in one plasmid.

Sequence Annotation Location Component / Role(s)
EcoRI site
RBS BD24
superfolding GFP
A-->G
T-->C
Stop
1,6
9,94
94,810
111,111
140,140
808,810
feature/misc sequence_feature
feature/rbs ribosome_entry_site
feature/protein CDS
sequence_alteration feature/mutation
sequence_alteration feature/mutation
feature/stop stop_codon
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J100201/1