BBa_J100206 1 BBa_J100206 tCloneRed RS10shift 2015-06-01T11:00:00Z 2015-08-31T04:08:24Z Wachsmuth et. al. "De novo design of a synthetic riboswitch that regulates transcription termination." Isolated pUC-IDT-Amp plasmid and inserted RS10shift riboswitch in place of the J119361 part. Removed J119361 sequence in between the two Bsa1 sites and inserted the DNA sequence for the RS10shift riboswitch. RS10shift has the same DNA sequence as the RS10 riboswitch, in addition to 19 added base pairs in after the U sequence. This was taken from the Wachsmuth et. al. paper. false false _578_ 0 23979 9 Not in stock false Extra Bsa1 site present in the pUC-IDT-Amp plasmid. false Helen Webster annotation2432883 1 BD18 C dog RBS range2432883 1 132 216 annotation2432588 1 P5 Promoter range2432588 1 1 36 annotation2432584 1 Riboswitch range2432584 1 41 127 annotation2432589 1 Spacer sequence range2432589 1 109 127 annotation2432882 1 RFP range2432882 1 217 897 BBa_J100206_sequence 1 ttgacaattaatcatccggctcgtaatttatgtggacgacaagtgataccagcatcgtcttgatgcccttggcagcacttcagaaatctctgaagtgctgttttttttaattcgagctccgtcgacagcgggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatggcttcctccgaagatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z