LOCUS       BBa_J100237             1799 bp    DNA     linear       08-Jun-2024
DEFINITION  Original Nitrate Biosensor (O)
ACCESSION   BBa_J100237
VERSION     BBa_J100237.1  
FEATURES             Location/Qualifiers
     CDS             1..720
                     /label=GFP Reverse
     CDS             1118..1799
                     /label=RFP
     promoter        755..1022
                     /label=narG promoter
     RBS             1032..1117
                     /label=BD18 bicistron RBS
ORIGIN
        1 ttattatttg tatagttcat ccatgccatg tgtaatccca gcagctgtta caaactcaag
       61 aaggaccatg tgatctctct tttcgttggg atctttcgaa agggcagatt gtgtggacag
      121 gtaatggttg tctggtaaaa ggacagggcc atcgccaatt ggagtatttt gttgataatg
      181 gtctgctagt tgaacgcttc catcttcaat gttgtgtcta attttgaagt taactttgat
      241 tccattcttt tgtttgtctg ccatgatgta tacattgtgt gagttatagt tgtattccaa
      301 tttgtgtcca agaatgtttc catcttcttt aaaatcaata ccttttaact cgattctatt
      361 aacaagggta tcaccttcaa acttgacttc agcacgtgtc ttgtagttcc cgtcatcttt
      421 gaaaaatata gttctttcct gtacataacc ttcgggcatg gcactcttga aaaagtcatg
      481 ctgtttcata tgatctgggt atctcgcaaa gcattgaaca ccataaccga aagtagtgac
      541 aagtgttggc catggaacag gtagttttcc agtagtgcaa ataaatttaa gggtaagttt
      601 tccgtatgtt gcatcacctt caccctctcc actgacagaa aatttgtgcc cattaacatc
      661 accatctaat tcaacaagaa ttgggacaac tccagtgaaa agttcttctc ctttacgcat
      721 ctagtatttc tcctctttaa tactagaggt cttccgactg ttatatggtt tatttcgccg
      781 gatttcatta agagccatta atatgttacc catggggaat actccttaat acccatctgc
      841 ataaaaatct taatagttta aataactaca ggtataaaac gtcttaattt acagtctgtt
      901 atgtggtggc tgttaattat cctaaagggg tatcttagga atttacttta tttttcatcc
      961 ccatcactct tgatcgttat caattcccac gctgtttcag agcgttacct tgcccttgcg
     1021 ggaagacaac taggggccca agttcactta aaaaggagat caacaatgaa agcaattttc
     1081 gtactgaaac atcttaatca tgcgacggag cgtttctaat ggcttcctcc gaagatgtta
     1141 tcaaagagtt catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg
     1201 aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag
     1261 ttaccaaagg tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg
     1321 gttccaaagc ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc
     1381 cggaaggttt caaatgggaa cgtgttatga acttcgaaga tggtggtgtt gttaccgtta
     1441 cccaggactc ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca
     1501 acttcccgtc cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg
     1561 aacgtatgta cccggaagat ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga
     1621 aagacggtgg tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc
     1681 agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac aacgaagatt
     1741 acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataa
//