LOCUS       BBa_J100240             2321 bp    DNA     linear       18-May-2024
DEFINITION  repClone with P5 Promoter
ACCESSION   BBa_J100240
VERSION     BBa_J100240.1  
FEATURES             Location/Qualifiers
     CDS             1..639
                     /label=TetR (C0041)
     misc_feature    640..763
                     /label=P5+BD24 (J119031+J119028)
     CDS             764..1483
                     /label=GFP - codon optimized
     RBS             1490..1501
                     /label=RBS B0034
     misc_feature    1512..1515
                     /label=sticky end
     promoter        1516..1551
                     /label=P5 Promoter (J119031)
     misc_feature    1552..1555
                     /label=sticky end
     RBS             1556..1643
                     /label=RBS BD18 (J119024)
     CDS             1641..2321
                     /label=RFP (E1010)
ORIGIN
        1 cggccgctac tagtattatt agctaccgct ttcacatttc agttgttttt ccagtccgca
       61 aataatcagt tctaaaccaa acagaaaggc aggttctgca ccctgatgat caaacagttc
      121 aatggcctga cgcagcagcg gaggcatgct atcggtggtc ggggtttcgc gttcttcttt
      181 tgcaacctga tgttcttgat cttccagaac acaacccagg gtaaaatgac caactgcgct
      241 cagtgcatac agtgcgtttt ccagactaaa accttgctga cacagaaatg ccagctgatt
      301 ttccagggtt tcatactgtt tttcggtcgg acgggtgccc agatgaactt ttgcaccatc
      361 acgatggctc agcagggcac aacgaaagct ttttgcatta ttacgcagaa aatcctgcca
      421 gctttcacct tccagcggac aaaaatgggt atgatgacga tccagcattt caattgccag
      481 tgcatccagc agtgcacgtt tgtttttaac atgccaatac agggtcggct gttcaacacc
      541 cagtttctgt gccagtttac gggtggtcag accttcaata ccaacttcat tcagcagttc
      601 cagtgcgcta ttaatcactt tgcttttatc cagacggctc attagaaacc gtccatcgca
      661 tgattaagat gtttcagtac gaaaattgct ttcattgttg atctcctttt taagtgaact
      721 tgggccctcc acataaatta cgagccggat gattaattgt caattattat ttgtatagtt
      781 catccatgcc atgtgtaatc ccagcagctg ttacaaactc aagaaggacc atgtgatctc
      841 tcttttcgtt gggatctttc gaaagggcag attgtgtgga caggtaatgg ttgtctggta
      901 aaaggacagg gccatcgcca attggagtat tttgttgata atggtctgct agttgaacgc
      961 ttccatcttc aatgttgtgt ctaattttga agttaacttt gattccattc ttttgtttgt
     1021 ctgccatgat gtatacattg tgtgagttat agttgtattc caatttgtgt ccaagaatgt
     1081 ttccatcttc tttaaaatca atacctttta actcgattct attaacaagg gtatcacctt
     1141 caaacttgac ttcagcacgt gtcttgtagt tcccgtcatc tttgaaaaat atagttcttt
     1201 cctgtacata accttcgggc atggcactct tgaaaaagtc atgctgtttc atatgatctg
     1261 ggtatctcgc aaagcattga acaccataac cgaaagtagt gacaagtgtt ggccatggaa
     1321 caggtagttt tccagtagtg caaataaatt taagggtaag ttttccgtat gttgcatcac
     1381 cttcaccctc tccactgaca gaaaatttgt gcccattaac atcaccatct aattcaacaa
     1441 gaattgggac aactccagtg aaaagttctt ctcctttacg catctagtat ttctcctctt
     1501 taattactag acgacttgac aattaatcat ccggctcgta atttatgtgg agcgggggcc
     1561 caagttcact taaaaaggag atcaacaatg aaagcaattt tcgtactgaa acatcttaat
     1621 catgcgacgg agcgtttcta atggcttcct ccgaagatgt tatcaaagag ttcatgcgtt
     1681 tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg
     1741 aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc
     1801 tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta
     1861 aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg
     1921 aacgtgttat gaacttcgaa gatggtggtg ttgttaccgt tacccaggac tcctccctgc
     1981 aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc
     2041 cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag
     2101 atggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg
     2161 acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca
     2221 aaaccgacat caaactggac atcacctccc acaacgaaga ttacaccatc gttgaacagt
     2281 acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata a
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