BBa_J100262

BBa_J100262 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J100262
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zachary Shaver
Date created: 2016-06-07 11:00:00
Date modified: 2016-06-08 10:13:19

rClone Red with Riboswitch E



Types
DnaRegion

Roles
Reporter

engineered_region

Sequences BBa_J100262_sequence (Version 1)

Description

This part is a modified form of the rClone Red plasmid (part BBa_J119384) that replaced the GFP section flanked by BsaI sites with a theophylline riboswitch, labeled riboswitch E, by Golden Gate Assembly (GGA). In the presence of theophylline, the cells should fluoresce red. In the absence of theophylline, the cells should not fluoresce.

Notes

/

Source

The sequence for riboswitch E was taken from the Gallivan et al. paper "A family of synthetic riboswitches adopts a kinetic trapping mechanism." The rClone Red plasmid used in GGA is part BBa_J119384.

Sequence Annotation Location Component / Role(s)
P5 promoter
Riboswitch E
Red Fluorescent Protein
1,36
41,94
101,781
feature/promoter promoter
ribosome_entry_site feature/rbs
CDS feature/cds
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J100262/1