LOCUS BBa_J100287 4981 bp DNA linear 13-May-2024 DEFINITION J100265 (pJC173b) with GFP replacing LuxAB ACCESSION BBa_J100287 VERSION BBa_J100287.1 FEATURES Location/Qualifiers misc_feature 955..1700 /label=SC101 Origin of Replication promoter 1784..1803 /label=T7 Promoter primer_bind 1784..1803 /label=T7 Primer Bind RBS 1825..1839 /label=SD8 RBS CDS 1840..3113 /label=geneIII CDS 3114..3830 /label=GFP CDS 3910..4770 /label=bla (AmpR) CDS 3913..4572 /label=AmpR promoter 4771..4965 /label=bla Promoter CDS 1..951 /label=RepA ORIGIN 1 tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag 61 ccatgagaac gaaccattga gatcatgctt actttgcatg tcactcaaaa attttgcctc 121 aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt 181 acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg 241 gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa 301 cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa 361 atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt 421 ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt 481 tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc 541 aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg 601 caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt 661 ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat 721 cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat 781 ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat 841 cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc 901 atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa 961 ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat gataattact 1021 agtccttttc ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact 1081 tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta 1141 tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga 1201 tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc 1261 gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac 1321 cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga 1381 gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg 1441 gtaaatggca ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag 1501 aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct 1561 gactttttgc tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta 1621 tcccgtgaca ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca 1681 ggcttacccg tcttactgtc aagaggacat ccggtcaaat aaaacgaaag gctcagtcga 1741 aagactgggc cttcgttttg ctgaggagac ttagggaccc tactaatacg actcactata 1801 gggagaaaga cctgcaggtg cagtaaggag gaaaaaaaaa tgaaaaaatt attattcgca 1861 attcctttag ttgttccttt ctattctcac tccgctgaaa ctgttgaaag ttgtttagca 1921 aaaccccata cagaaaattc atttactaac gtctggaaag acgacaaaac tttagatcgt 1981 tacgctaact atgagggctg tctgtggaat gctacaggcg ttgtagtttg tactggtgac 2041 gaaactcagt gttacggtac atgggttcct attgggcttg ctatccctga aaatgagggt 2101 ggtggctctg agggtggcgg ttctgagggt ggcggttctg agggtggcgg tactaaacct 2161 cctgagtacg gtgatacacc tattccgggc tatacttata tcaaccctct cgacggcact 2221 tatccgcctg gtactgagca aaaccccgct aatcctaatc cttctcttga ggagtctcag 2281 cctcttaata ctttcatgtt tcagaataat aggttccgaa ataggcaggg ggcattaact 2341 gtttatacgg gcactgttac tcaaggcact gaccccgtta aaacttatta ccagtacact 2401 cctgtatcat caaaagccat gtatgacgct tactggaacg gtaaattcag agactgcgct 2461 ttccattctg gctttaatga ggatccattc gtttgtgaat atcaaggcca atcgtctgac 2521 ctgcctcaac ctcctgtcaa tgctggcggc ggctctggtg gtggttctgg tggcggctct 2581 gagggtggtg gctctgaggg tggcggttct gagggtggcg gctctgaggg aggcggttcc 2641 ggtggtggct ctggttccgg tgattttgat tatgaaaaga tggcaaacgc taataagggg 2701 gctatgaccg aaaatgccga tgaaaacgcg ctacagtctg acgctaaagg caaacttgat 2761 tctgtcgcta ctgattacgg tgctgctatc gatggtttca ttggtgacgt ttccggcctt 2821 gctaatggta atggtgctac tggtgatttt gctggctcta attcccaaat ggctcaagtc 2881 ggtgacggtg ataattcacc tttaatgaat aatttccgtc aatatttacc ttccctccct 2941 caatcggttg aatgtcgccc ttttgtcttt ggcgctggta aaccttacga gttcagtatc 3001 gactgcgata agatcaacct gttccgcggt gtctttgcgt ttcttttata tgttgccacc 3061 tttatgtatg tattttctac gtttgctaac atactgcgta ataaggagtc ttaatgcgta 3121 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta 3181 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta 3241 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta 3301 ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact 3361 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg 3421 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa 3481 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat 3541 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag 3601 ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc 3661 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca 3721 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga tcacatggtc cttcttgagt 3781 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa acttaattaa 3841 cggcactcct cagcaaatat aatgaccctc ttgataaccc aagagggcat tttttaatgc 3901 ccatggcgtt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 3961 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 4021 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 4081 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 4141 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 4201 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 4261 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 4321 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 4381 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 4441 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 4501 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 4561 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 4621 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 4681 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 4741 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 4801 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 4861 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 4921 tatcatgaca ttaacctata aaaataggcg tatcacgagg cccttaggcc aaataggccg 4981 t //