LOCUS BBa_J100288 6178 bp DNA linear 01-Jun-2024 DEFINITION pJC173b with gIII neg ACCESSION BBa_J100288 VERSION BBa_J100288.1 FEATURES Location/Qualifiers CDS 1..951 /label=RepA misc_feature 955..1700 /label=SC101 Origin of Replication promoter 1784..1803 /label=T7 promoter primer_bind 1784..1803 /label=T7 RBS 1825..1839 /label=SD8 RBS CDS 1840..2903 /label=Gene III neg CDS 2904..5027 /label=LuxAB CDS 5110..5769 /label=AmpR promoter 5968..6162 /label=bla promoter ORIGIN 1 tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag 61 ccatgagaac gaaccattga gatcatgctt actttgcatg tcactcaaaa attttgcctc 121 aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt 181 acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg 241 gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa 301 cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa 361 atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt 421 ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt 481 tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc 541 aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg 601 caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt 661 ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat 721 cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat 781 ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat 841 cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc 901 atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa 961 ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat gataattact 1021 agtccttttc ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact 1081 tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta 1141 tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga 1201 tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc 1261 gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac 1321 cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga 1381 gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg 1441 gtaaatggca ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag 1501 aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct 1561 gactttttgc tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta 1621 tcccgtgaca ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca 1681 ggcttacccg tcttactgtc aagaggacat ccggtcaaat aaaacgaaag gctcagtcga 1741 aagactgggc cttcgttttg ctgaggagac ttagggaccc tactaatacg actcactata 1801 gggagaaaga cctgcaggtg cagtaaggag gaaaaaaaaa tgaaaaaatt attattcgca 1861 attcctttag ttgttccttt ctattctcac tccgctgaaa ctgttgaaag ttgtttagca 1921 aaaccccata cagaaaattc atttactaac gtctggaaag acgacaaaac tttagatcgt 1981 tacgctaact atgagggctg tctgtggaat gctacaggcg ttgtagtttg tactggtgac 2041 gaaactcagt gttacggtac atgggttcct attgggcttg ctatccctga aaatgagggt 2101 ggtggctctg agggtggcgg ttctgagggt ggcggttctg agggtggcgg tactaaacct 2161 cctgagtacg gtgatacacc tattccgggc tatacttata tcaaccctct cgacggcact 2221 tatccgcctg gtactgagca aaaccccgct aatcctaatc cttctcttga ggagtctcag 2281 cctcttaata ctttcatgtt tcagaataat aggttccgaa ataggcaggg ggcattaact 2341 gtttatacgg gcactgttac tcaaggcact gaccccgtta aaacttatta ccagtacact 2401 cctgtatcat caaaagccat gtatgacgct tactggaacg gtaaattcag agactgcgct 2461 ttccattctg gctttaatga ggatccattc gtttgtgaat atcaaggcca atcgtctgac 2521 ctgcctcaac ctcctgtcaa tgctggcggc ggctctggtg gtggttctgg tggcggctct 2581 gagggtggtg gctctgaggg tggcggttct gagggtggcg gctctgaggg aggcggttcc 2641 ggtggtggct cttcccaaat ggctcaagtc ggtgacggtg ataattcacc tttaatgaat 2701 aatttccgtc aatatttacc ttccctccct caatcggttg aatgtcgccc ttttgtcttt 2761 ggcgctggta aaccttacga gttcagtatc gactgcgata agatcaacct gttccgcggt 2821 gtctttgcgt ttcttttata tgttgccacc tttatgtatg tattttctac gtttgctaac 2881 atactgcgta ataaggagtc ttaatgaaat ttggaaactt tttgcttaca taccaacctc 2941 cccaattttc ccaaacagag gtaatgaaac gtttggttaa attaggtcgc atctctgagg 3001 agtgtggttt tgataccgta tggttactgg agcatcattt cacggagttt ggtttgcttg 3061 gtaaccctta tgtcgctgct gcatatttac ttggcgcgac taaaaaattg aatgtaggaa 3121 ctgccgctat tgttcttccc acagcccatc cagtacgcca acttgaagat gtgaatttat 3181 tggatcaaat gtcaaaagga cgatttcggt ttggtatttg ccgagggctt tacaacaagg 3241 actttcgcgt attcggcaca gatatgaata acagtcgcgc cttagcggaa tgctggtacg 3301 ggctgataaa gaatggcatg acagagggat atatggaagc tgataatgaa catatcaagt 3361 tccataaggt aaaagtaaac cccgcggcgt atagcagagg tggcgcaccg gtttatgtgg 3421 tggctgaatc agcttcgacg actgagtggg ctgctcaatt tggcctaccg atgatattaa 3481 gttggattat aaatactaac gaaaagaaag cacaacttga gctttataat gaagtggctc 3541 aagaatatgg gcacgatatt cataatatcg accattgctt atcatatata acatctgtag 3601 atcatgactc aattaaagcg aaagagattt gccggaaatt tctggggcat tggtatgatt 3661 cttatgtgaa tgctacgact atttttgatg attcagacca aacaagaggt tatgatttca 3721 ataaagggca gtggcgtgac tttgtattaa aaggacataa agatactaat cgccgtattg 3781 attacagtta cgaaatcaat cccgtgggaa cgccgcagga atgtattgac ataattcaaa 3841 aagacattga tgctacagga atatcaaata tttgttgtgg atttgaagct aatggaacag 3901 tagacgaaat tattgcttcc atgaagctct tccagtctga tgtcatgcca tttcttaaag 3961 aaaaacaacg ttcgctatta tattatggcg gtggcggtag cggcggtggc ggtagcggcg 4021 gtggcggtag cggcggtggc ggtagcaaat ttggattgtt cttccttaac ttcatcaatt 4081 caacaactgt tcaagaacag agtatagttc gcatgcagga aataacggag tatgttgata 4141 agttgaattt tgaacagatt ttagtgtatg aaaatcattt ttcagataat ggtgttgtcg 4201 gcgctcctct gactgtttct ggttttctgc tcggtttaac agagaaaatt aaaattggtt 4261 cattaaatca catcattaca actcatcatc ctgtccgcat agcggaggaa gcttgcttat 4321 tggatcagtt aagtgaaggg agatttattt tagggtttag tgattgcgaa aaaaaagatg 4381 aaatgcattt ttttaatcgc ccggttgaat atcaacagca actatttgaa gagtgttatg 4441 aaatcattaa cgatgcttta acaacaggct attgtaatcc agataacgat ttttatagct 4501 tccctaaaat atctgtaaat ccccatgctt atacgccagg cggacctcgg aaatatgtaa 4561 cagcaaccag tcatcatatt gttgagtggg cggccaaaaa aggtattcct ctcatcttta 4621 agtgggatga ttctaatgat gttagatatg aatatgctga aagatataaa gccgttgcgg 4681 ataaatatga cgttgaccta tcagagatag accatcagtt aatgatatta gttaactata 4741 acgaagatag taataaagct aaacaagaga cgcgtgcatt tattagtgat tatgttcttg 4801 aaatgcaccc taatgaaaat ttcgaaaata aacttgaaga aataattgca gaaaacgctg 4861 tcggaaatta tacggagtgt ataactgcgg ctaagttggc aattgaaaag tgtggtgcga 4921 aaagtgtatt gctgtccttt gaaccaatga atgatttgat gagccaaaaa aatgtaatca 4981 atattgttga tgataatatt aagaagtacc acacggaata tacctaaact taattaacgg 5041 cactcctcag caaatataat gaccctcttg ataacccaag agggcatttt ttaatgccca 5101 tggcgtttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 5161 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 5221 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 5281 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 5341 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 5401 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 5461 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 5521 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 5581 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 5641 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 5701 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 5761 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 5821 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 5881 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 5941 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 6001 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 6061 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 6121 catgacatta acctataaaa ataggcgtat cacgaggccc ttaggccaaa taggccgt //