BBa_J100304 1 BBa_J100304 NPT-II 2016-09-07T11:00:00Z 2016-09-08T01:50:23Z The promoter was found in 'Letters in Applied Microbiology' titled 'AiiA-mediated quorum quenching does not affect virulence or toxoflavin expression in Burkholderia glumae SL2376'. The NPT-II promoter is originally short, with fewer than 80 bases in its entirety. The part of the promoter has been trimmed to within twelve bases of the -10 and -35 regions. This promoter is constitutive. The -10 region is located at the 52nd base in the promoter, and the -35 region is located at the 27th base. The promoter has the ability to be cut by the EcoRI restriction enzyme. false false _578_ 33873 33873 9 false The promoter had to be manipulated to fewer than 60 base pairs. false India Little annotation2482709 1 -35 box range2482709 1 14 20 annotation2482708 1 -10 box range2482708 1 36 42 BBa_J100304_sequence 1 ggcgccctctggtaaggttgggaagccctgcaaatgacagtaaagaagctt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z