LOCUS       BBa_J10052              1016 bp    DNA     linear       01-Jun-2024
DEFINITION  weak promoter + med RBS + lacZa.GFP fusion
ACCESSION   BBa_J10052
VERSION     BBa_J10052.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23113
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     CDS             63..242
                     /label=lacZa
     misc_feature    243..299
                     /label=linker
     CDS             300..1016
                     /label=GFP
     misc_feature    63..1016
                     /label=BBa_E0051
ORIGIN
        1 ctgatggcta gctcagtcct agggattatg ctagctacta gagtcacaca ggaaagtact
       61 agatgaccat gattacggat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
      121 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
      181 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
      241 gcgcgggcgg cagcgaaggc ggcggcagcg aacatcatca tcatcatcat ggcagcgaac
      301 gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg
      361 ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac
      421 ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca acacttgtca
      481 ctaccctgac ctatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg
      541 actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag
      601 atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata
      661 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg
      721 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca
      781 aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt
      841 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt
      901 ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg
      961 agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa taataa
//