LOCUS       BBa_J107041             1777 bp    DNA     linear       17-May-2024
DEFINITION  heat-inducible TetR (with very strong RBS)
ACCESSION   BBa_J107041
VERSION     BBa_J107041.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23114
     misc_feature    36..43
                     /label=BBa_G0002
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             56..769
                     /label=BBa_J107033
     misc_feature    770..777
                     /label=BBa_G0002
     stem_loop       785..804
                     /label=T7 TE
     misc_feature    811..811
                     /label=stop
     polyA_site      805..818
                     /label=polya
     misc_feature    778..818
                     /label=BBa_B0012
     terminator      778..818
                     /label=BBa_B0012
     misc_feature    819..826
                     /label=BBa_G0002
     stem_loop       839..861
                     /label=stem_loop
     misc_feature    827..872
                     /label=BBa_B0011
     terminator      827..872
                     /label=BBa_B0011
     misc_feature    873..880
                     /label=BBa_G0002
     promoter        918..923
                     /label=-10
     promoter        895..900
                     /label=-35
     misc_feature    905..921
                     /label=OR1
     misc_feature    881..897
                     /label=OR2
     misc_feature    881..929
                     /label=BBa_R0051
     promoter        881..929
                     /label=BBa_R0051
     misc_feature    1..929
                     /label=BBa_J107034
     misc_feature    942..945
                     /label=conserved
     RBS             938..949
                     /label=BBa_B0034
     CDS             959..1575
                     /label=tetR
     misc_feature    1576..1609
                     /label=SsrA
     misc_feature    1616..1640
                     /label=Help:Barcodes
     CDS             956..1615
                     /label=BBa_C0040
     stem_loop       1660..1703
                     /label=stem_loop
     misc_feature    1649..1728
                     /label=BBa_B0010
     terminator      1649..1728
                     /label=BBa_B0010
     stem_loop       1744..1763
                     /label=T7 TE
     misc_feature    1770..1770
                     /label=stop
     polyA_site      1764..1777
                     /label=polya
     misc_feature    1737..1777
                     /label=BBa_B0012
     terminator      1737..1777
                     /label=BBa_B0012
     misc_feature    938..1777
                     /label=BBa_P0440
ORIGIN
        1 tttatggcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaaatgag
       61 cacaaaaaag aaaccattaa cacaagagca gcttgaggac gcacgtcgcc ttaaagcaat
      121 ttatgaaaaa aagaaaaatg aacttggctt atcccaggaa tctgtcgcag acaagatggg
      181 gatggggcag tcaggcgttg gtgctttatt taatggcatc aatgcattaa atgcttataa
      241 cgccgcattg cttacaaaaa ttctcaaagt tagcgttgaa gaatttagcc cttcaatcgc
      301 cagagaaatc tacgagatgt atgaagcggt tagtatgcag ccgtcactta gaagtgagta
      361 tgagtaccct gttttttctc atgttcaggc agggatgttc tcacctaagc ttagaacctt
      421 taccaaaggt gatgcggaga gatgggtaag cacaaccaaa aaagccagtg attctgcatt
      481 ctggcttgag gttgaaggta attccatgac cgcaccaaca ggctccaagc caagctttcc
      541 tgacggaatg ttaattctcg ttgaccctga gcaggctgtt gagccaggtg atttctgcat
      601 agccagactt gggggtgatg agtttacctt caagaaactg atcagggata gcggtcaggt
      661 gtttttacaa ccactaaacc cacagtaccc aatgatccca tgcaatgaga gttgttccgt
      721 tgtggggaaa gttatcgcta gtcagtggcc tgaagagacg tttggctgat actagagtca
      781 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagagag aatataaaaa
      841 gccagattat taatccggct tttttattat tttactagag taacaccgtg cgtgttgact
      901 attttacctc tggcggtgat aatggttgct actagagaaa gaggagaaat actagatgtc
      961 cagattagat aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat
     1021 cgaaggttta acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta
     1081 ttggcatgta aaaaataagc gggctttgct cgacgcctta gccattgaga tgttagatag
     1141 gcaccatact cacttttgcc ctttagaagg ggaaagctgg caagattttt tacgtaataa
     1201 cgctaaaagt tttagatgtg ctttactaag tcatcgcgat ggagcaaaag tacatttagg
     1261 tacacggcct acagaaaaac agtatgaaac tctcgaaaat caattagcct ttttatgcca
     1321 acaaggtttt tcactagaga atgcattata tgcactcagc gctgtggggc attttacttt
     1381 aggttgcgta ttggaagatc aagagcatca agtcgctaaa gaagaaaggg aaacacctac
     1441 tactgatagt atgccgccat tattacgaca agctatcgaa ttatttgatc accaaggtgc
     1501 agagccagcc ttcttattcg gccttgaatt gatcatatgc ggattagaaa aacaacttaa
     1561 atgtgaaagt gggtccgctg caaacgacga aaactacgct ttagtagctt aataacactg
     1621 atagtgctag tgtagatcac tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     1681 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     1741 actggctcac cttcgggtgg gcctttctgc gtttata
//