LOCUS BBa_J107058 3279 bp DNA linear 09-May-2024 DEFINITION BioBrick integrative vector targeting aspA locus ACCESSION BBa_J107058 VERSION BBa_J107058.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Suffix misc_feature 2..7 /label=SpeI misc_feature 16..21 /label=PstI misc_feature 9..16 /label=NotI misc_feature 1..21 /label=BBa_G00001 stem_loop 32..63 /label=stem loop misc_feature 25..64 /label=identical to E. coli MG1655 between tonB and yciA terminator 22..90 /label=BBa_B0054 primer_bind 91..110 /label=VR misc_feature 91..110 /label=BBa_G00102 stem_loop 124..143 /label=stem_loop terminator 111..151 /label=BBa_B0062 misc_feature 152..187 /label=BBa_J72001 misc_feature 188..194 /label=BBa_K300994 misc_feature 195..600 /label=BBa_K300998 misc_feature 601..608 /label=BBa_G0001 misc_feature 609..649 /label=B0012 misc_feature 658..737 /label=B0010 terminator 609..737 /label=BBa_B0025 misc_feature 738..745 /label=BBa_G0001 CDS 746..1408 /label=cmR(rev) misc_feature 746..1514 /label=BBa_K300999 misc_feature 1515..1521 /label=BBa_K300995 misc_feature 1522..1557 /label=BBa_J72001 misc_feature 1558..1564 /label=BBa_G0005 CDS 1565..3082 /label=BBa_J107059 misc_feature 3083..3087 /label=BBa_J107060 stem_loop 3096..3130 /label=stem_loop terminator 3088..3159 /label=BBa_B0053 primer_bind 3160..3179 /label=VF2 primer 3160..3179 /label=BBa_G00100 stem_loop 3215..3236 /label=stem loop from bacteriophage T3 misc_feature 3207..3245 /label=identical to bacteriophage T3 terminator 3180..3257 /label=BBa_B0055 misc_feature 3258..3263 /label=EcoRI site misc_feature 3273..3278 /label=XbaI site misc_feature 3258..3279 /label=Prefix misc_feature 3264..3271 /label=NotI site misc_feature 3258..3279 /label=BBa_G00000 ORIGIN 1 tactagtagc ggccgctgca gattagcaga aagtcaaaag cctccgaccg gaggcttttg 61 actaaaactt cccttggggt tatcattggg gctcactcaa aggcggtaat cagataaaaa 121 aaatccttag ctttcgctaa ggatgatttc tgcgaagttc ctatactttc tagagaatag 181 gaacttccct agtagcagtt caacctgttg atagtacgta ctaagctctc atgtttcacg 241 tactaagctc tcatgtttaa cgtactaagc tctcatgttt aacgaactaa accctcatgg 301 ctaacgtact aagctctcat ggctaacgta ctaagctctc atgtttcacg tactaagctc 361 tcatgtttga acaataaaat taatataaat cagcaactta aatagcctct aaggttttaa 421 gttttataag aaaaaaaaga atatataagg cttttaaagc ttttaaggtt taacggttgt 481 ggacaacaag ccagggatgt aacgcactga gaagccctta gagcctctca aagcaatttt 541 gagtgacaca ggaacactta acggctgaca tgggaattag ccatgggccc gtgcgaatca 601 ctctagtata taaacgcaga aaggcccacc cgaaggtgag ccagtgtgac tctagtagag 661 agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg 721 ttttatttga tgcctggctc tagtattatt acgccccgcc ctgccactca tcacagtact 781 gttgtaattc attaagcatg cggccgacat ggaagccatc acaaacggca tgatgaacct 841 ggatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atcgtgaaaa 901 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc 961 agggattggc tgagacaaaa aacatattct caataaaccc tttagggaaa taggccaggt 1021 tttcaccgta acacgccaca tcttgcgaat atatgtggag aaactgccgg aaatcgtcgt 1081 ggtattcact ccagagggac gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag 1141 ggtgaacact atcccagatc accagctcac cgtctttcat ggccatacga aactccgggt 1201 gagcgttcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt 1261 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt 1321 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg 1381 tggtatatcc cgtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata 1441 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta 1501 cgtgcccgat caacctctag ggcgaagttc ctatactttc tagagaatag gaacttcgct 1561 agtattatta agctactaaa gcgtagtttt cgtcgtttgc agcctgttcg ctttcatcag 1621 tatagcgttt tgctttgtaa gccgggtgca tcagattctg tacggagaaa atatcgtcaa 1681 gttccgcttc agtcaacaga ccgcgttcca gaacgacttc acgtacactc ttaccggttt 1741 cggcacagat tttacccacg atgtcaccgt tgtggtgacc gatgaacggg ttcaggtaag 1801 taacgatacc gatagagttg taaacgtaac cttcgcacac ttctttgtta gcagtgatgc 1861 cgttaatgca tttttccagc aggttgtagc aagcgttggt cagaatgtga acggattcga 1921 acatggcctg gccaatgacc ggctccataa cgttcaattg cagctgacct gcttctgctg 1981 ccatggtaac agtggtgtcg ttaccgatga ctttgaagca tacctggtta accacttccg 2041 gaacaaccgg gtttacttta gctggcatga tggaagagcc cgcttgcagt tccggcaggt 2101 tgatctcgtt caggccggca cgtgggcctg aagagagcaa gcgcaggtcg ttacagattt 2161 tggacatctt cacagccagg cgtttcagcg cgccgtgaac cataacataa gcgccgcagt 2221 cagaggtcgc ttcgatcagg tcttcagccg gtacgcatgg gaagccagta acttcagcca 2281 gttttttcac tgccagcgga gagtactctt tcggcgtgtt cagaccagta ccgattgctg 2341 ttgcaccaag gttaacttcc agcagcagtt cagcggtacg ttggatgttt ttcacttctt 2401 ctttcagcag gatgctgaaa gcgcgaaatt cctgaccgag ggtcatcggt actgcgtctt 2461 gcagctgggt acgacccatt ttcaggatgt cctgaaattc gacagcttta cgttcaaagc 2521 cttcacgcag ttggttaatc gcatctacca gcttaatcag ggaagagtaa actgcgatac 2581 ggaaaccggt cgggtaggcg tcgttagtgg actgacattt gttaacatgg tcgttcgggt 2641 tcaggtactg atattcacct ttttggtgac ccatcagttc cagaccgata ttggccagca 2701 cttcgttggt gttcatgttt acggaagtac ctgcgccgcc ctggtagacg tctaccggga 2761 actgatccat gcattttccg ttgttcagga cttcatcaca tgcggcaatg atggcattcg 2821 ctacactttt aggaatggtt tgcagctctt tgtttgccat agctgcggct tttttaacca 2881 ttaccatacc gcgaacaaat tcaggaatat cactgatttt gttgttgctg atatagaagt 2941 tttcaatcgc tctcagagtg tgaacaccat agtaggcatc agctggaact tccctggtac 3001 ccaacagatc ttcttcgata cgaatgttgt ttgacatgtg aaccttcttt ttcaagctgc 3061 caatgatttg ctttaaacac atctagctcc ggcaaaaaaa cgggcaaggt gtcaccaccc 3121 tgcccttttt ctttaaaacc gaaaagatta cttcgcgttt gccacctgac gtctaagaaa 3181 aggaatattc agcaatttgc ccgtgccgaa gaaaggccca cccgtgaagg tgagccagtg 3241 agttgattgc tacgtaagaa ttcgcggccg cttctagag //