LOCUS       BBa_J110001             1750 bp    DNA     linear       11-May-2024
DEFINITION  pulse generator/feedback controller
ACCESSION   BBa_J110001
VERSION     BBa_J110001.1  
FEATURES             Location/Qualifiers
     misc_feature    1..97
                     /label=BBa_R0065
     misc_feature    6..25
                     /label=Lux Box
     promoter        47..52
                     /label=-10
     misc_feature    57..73
                     /label=OR2 cI
     promoter        58..58
                     /label=lux p(R) start
     promoter        71..76
                     /label=-35
     misc_feature    81..97
                     /label=OR1 cI
     promoter        94..97
                     /label=-10
     promoter        1..97
                     /label=BBa_R0065
     RBS             106..118
                     /label=RBS-3\Weak
     RBS             112..115
                     /label=RBS
     misc_feature    106..118
                     /label=BBa_B0032
     RBS             106..118
                     /label=BBa_B0032
     CDS             125..841
                     /label=cI lambda
     misc_feature    842..866
                     /label=Help:Barcodes
     CDS             125..866
                     /label=BBa_I9200
     RBS             875..887
                     /label=RBS-3\Weak
     RBS             881..884
                     /label=RBS
     misc_feature    875..887
                     /label=BBa_B0032
     RBS             875..887
                     /label=BBa_B0032
     CDS             894..1613
                     /label=GFP protein
     CDS             894..1613
                     /label=BBa_E0040
     misc_feature    1622..1662
                     /label=B0012
     misc_feature    1671..1750
                     /label=B0010
     terminator      1622..1750
                     /label=BBa_B0025
ORIGIN
        1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac
       61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagtcaca caggaaagta
      121 ctagatgagc actaaaaaga aaccacttac tcaagagcag ttagaggacg cccgtcgctt
      181 aaaagccatt tatgaaaaaa agaaaaatga attaggcctt tcccaggaat cagtcgccga
      241 caagatgggg atggggcagt cgggcgttgg tgctcttttt aatggcatca atgcccttaa
      301 tgcttataac gcggccctct tagccaaaat tttgaaagtt agcgttgaag aatttagccc
      361 ttcgatcgcg cgagaaatct acgagatgta tgaagcagtt agtatgcagc cgtcgttacg
      421 aagtgagtat gagtaccctg ttttttcaca tgttcaggcc gggatgttct cgcctgagtt
      481 acgaaccttt accaaaggtg atgcagagcg atgggtaagc actaccaaaa aagcgagtga
      541 ttcagccttc tggttagagg ttgaaggtaa ttccatgacc gccccaactg gctccaagcc
      601 aagctttcct gacggaatgc ttattttggt tgaccctgag caggctgttg agccaggtga
      661 tttctgcata gcgcgattag ggggtgatga gtttaccttc aagaaactga tcagggatag
      721 cggtcaggtg tttcttcaac cactaaaccc acagtaccca atgatcccat gcaatgagag
      781 ttgttccgtt gtggggaaag ttatcgctag tcagtggcct gaagagacgt ttggctaata
      841 actctgatag tgctagtgta gatctctact agagtcacac aggaaagtac tagatgcgta
      901 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
      961 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
     1021 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta
     1081 ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact
     1141 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg
     1201 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa
     1261 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat
     1321 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag
     1381 ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc
     1441 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca
     1501 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt
     1561 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa taatactaga
     1621 gtataaacgc agaaaggccc acccgaaggt gagccagtgt gactctagta gagagcgttc
     1681 accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt
     1741 tgatgcctgg
//