LOCUS       BBa_J11002              2489 bp    DNA     linear       13-May-2024
DEFINITION  improved pulse generator/PI controller
ACCESSION   BBa_J11002
VERSION     BBa_J11002.1  
FEATURES             Location/Qualifiers
     misc_feature    1..97
                     /label=BBa_R0065
     misc_feature    6..25
                     /label=Lux Box
     promoter        47..52
                     /label=-10
     misc_feature    57..73
                     /label=OR2 cI
     promoter        58..58
                     /label=lux p(R) start
     promoter        71..76
                     /label=-35
     misc_feature    81..97
                     /label=OR1 cI
     promoter        94..97
                     /label=-10
     promoter        1..97
                     /label=BBa_R0065
     RBS             106..118
                     /label=RBS-3\Weak
     RBS             112..115
                     /label=RBS
     misc_feature    106..118
                     /label=BBa_B0032
     RBS             106..118
                     /label=BBa_B0032
     CDS             125..841
                     /label=cI lambda
     misc_feature    842..866
                     /label=Help:Barcodes
     CDS             125..866
                     /label=BBa_I9200
     RBS             875..887
                     /label=RBS-3\Weak
     RBS             881..884
                     /label=RBS
     misc_feature    875..887
                     /label=BBa_B0032
     RBS             875..887
                     /label=BBa_B0032
     CDS             894..1541
                     /label=cII p22
     misc_feature    1542..1574
                     /label=LVA
     misc_feature    1575..1580
                     /label=stop
     misc_feature    894..1580
                     /label=BBa_C0053
     misc_feature    1581..1605
                     /label=Help:Barcodes
     CDS             894..1580
                     /label=BBa_C0053
     RBS             1614..1626
                     /label=RBS-3\Weak
     RBS             1620..1623
                     /label=RBS
     misc_feature    1614..1626
                     /label=BBa_B0032
     RBS             1614..1626
                     /label=BBa_B0032
     CDS             1633..2352
                     /label=GFP protein
     CDS             1633..2352
                     /label=BBa_E0040
     misc_feature    2361..2401
                     /label=B0012
     misc_feature    2410..2489
                     /label=B0010
     terminator      2361..2489
                     /label=BBa_B0025
ORIGIN
        1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac
       61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagtcaca caggaaagta
      121 ctagatgagc actaaaaaga aaccacttac tcaagagcag ttagaggacg cccgtcgctt
      181 aaaagccatt tatgaaaaaa agaaaaatga attaggcctt tcccaggaat cagtcgccga
      241 caagatgggg atggggcagt cgggcgttgg tgctcttttt aatggcatca atgcccttaa
      301 tgcttataac gcggccctct tagccaaaat tttgaaagtt agcgttgaag aatttagccc
      361 ttcgatcgcg cgagaaatct acgagatgta tgaagcagtt agtatgcagc cgtcgttacg
      421 aagtgagtat gagtaccctg ttttttcaca tgttcaggcc gggatgttct cgcctgagtt
      481 acgaaccttt accaaaggtg atgcagagcg atgggtaagc actaccaaaa aagcgagtga
      541 ttcagccttc tggttagagg ttgaaggtaa ttccatgacc gccccaactg gctccaagcc
      601 aagctttcct gacggaatgc ttattttggt tgaccctgag caggctgttg agccaggtga
      661 tttctgcata gcgcgattag ggggtgatga gtttaccttc aagaaactga tcagggatag
      721 cggtcaggtg tttcttcaac cactaaaccc acagtaccca atgatcccat gcaatgagag
      781 ttgttccgtt gtggggaaag ttatcgctag tcagtggcct gaagagacgt ttggctaata
      841 actctgatag tgctagtgta gatctctact agagtcacac aggaaagtac tagatgaata
      901 cacaattgat gggtgagcgt attcgcgctc gaagaaaaaa actcaagatt agacaagccg
      961 ctcttggtaa gatggtggga gtgtctaatg ttgcaatatc gcaatgggag cgctcggaga
     1021 ctgagccaaa tggggagaac ctgttggcac tttcgaaggc tcttcagtgc tcccctgact
     1081 atttgctgaa aggagattta agccagacaa acgttgccta tcatagtagg catgagccaa
     1141 gaggatcata ccctcttatc agttgggtaa gcgcagggca atggatggaa gctgtagaac
     1201 cttatcacaa gcgcgcgata gagaactggc acgacaccac tgtagattgt tcagaagatt
     1261 cattttggct tgatgtccaa ggtgactcta tgacagcacc ggcagggtta agcattccag
     1321 aaggaatgat aattctggtt gatcccgaag tcgaaccaag aaacggcaag ctggttgttg
     1381 caaaattaga aggtgaaaac gaggccacat tcaaaaaatt agttatggat gcaggccgaa
     1441 agtttttaaa accattaaac ccacaatatc cgatgataga aatcaacgga aactgcaaaa
     1501 tcattggcgt agttgttgac gcaaaactcg caaatcttcc agctgcaaac gacgaaaact
     1561 acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta gagtcacaca
     1621 ggaaagtact agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
     1681 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
     1741 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
     1801 tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat
     1861 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
     1921 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt
     1981 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
     2041 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa
     2101 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt
     2161 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
     2221 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
     2281 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
     2341 tacaaataat aatactagag tataaacgca gaaaggccca cccgaaggtg agccagtgtg
     2401 actctagtag agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga
     2461 ctgagccttt cgttttattt gatgcctgg
//