LOCUS       BBa_J11005               952 bp    DNA     linear       11-May-2024
DEFINITION  temperature sensor - low temperature color
ACCESSION   BBa_J11005
VERSION     BBa_J11005.1  
FEATURES             Location/Qualifiers
     misc_feature    9..25
                     /label=OR1
     misc_feature    33..49
                     /label=OR2
     promoter        48..53
                     /label=-35
     misc_feature    59..65
                     /label=mutate to obliterate OR3
     promoter        71..76
                     /label=-10
     promoter        1..82
                     /label=BBa_I12007
     RBS             91..103
                     /label=RBS-3\Weak
     RBS             97..100
                     /label=RBS
     misc_feature    91..103
                     /label=BBa_B0032
     RBS             91..103
                     /label=BBa_B0032
     CDS             110..784
                     /label=mrfp1
     misc_feature    791..815
                     /label=Help:Barcodes
     CDS             110..790
                     /label=BBa_E1010
     stem_loop       835..878
                     /label=stem_loop
     misc_feature    824..903
                     /label=BBa_B0010
     terminator      824..903
                     /label=BBa_B0010
     stem_loop       919..938
                     /label=T7 TE
     misc_feature    945..945
                     /label=stop
     polyA_site      939..952
                     /label=polya
     misc_feature    912..952
                     /label=BBa_B0012
     terminator      912..952
                     /label=BBa_B0012
ORIGIN
        1 gcaaccatta tcaccgccag aggtaaaata gtcaacacgc acggtgttag atatttataa
       61 atagtggtga tagatttaac gttactagag tcacacagga aagtactaga tggcttcctc
      121 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg
      181 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc
      241 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca
      301 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa
      361 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt
      421 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact
      481 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga
      541 agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg
      601 tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa
      661 aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca
      721 caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg
      781 tgcttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa
      841 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
      901 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//